Bioprinting induced cell damage in cellular micro-fluidic channel fabrication

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Bioprinting, or layer by layer additive tissue fabrication, is a revolutionary concept recently emerged as an interdisciplinary effort to produce three-dimensional living organ for clinical application. Among many challenges, it was agreed that inclusion of vascular system is critical for maintaining the viability and functionality of relatively thick 3D bioprinted tissue constructs. Our previous research addressed the printability of novel vessel-like micro-fluidic channels with alginate hydrogel and co-axial nozzles. Here, we further investigated the influence of bioprinting parameters on cartilage progenitor cells (CPCs) survival during and post printing. The results of this study revealed that quantifiable cell death could be induced by varying dispensing pressure, co-axial nozzle geometry, biomaterial concentration. However, damaged cells were able to recover during incubation, as well as undergo proliferation to certain extend. These findings may serve as a guideline for optimizing our system as well as predict cell damage in future studies.

Original languageEnglish (US)
Title of host publicationASME 2013 International Manufacturing Science and Engineering Conference Collocated with the 41st North American Manufacturing Research Conference, MSEC 2013
DOIs
StatePublished - Dec 18 2013
EventASME 2013 International Manufacturing Science and Engineering Conference Collocated with the 41st North American Manufacturing Research Conference, MSEC 2013 - Madison, WI, United States
Duration: Jun 10 2013Jun 14 2013

Publication series

NameASME 2013 International Manufacturing Science and Engineering Conference Collocated with the 41st North American Manufacturing Research Conference, MSEC 2013
Volume1

Other

OtherASME 2013 International Manufacturing Science and Engineering Conference Collocated with the 41st North American Manufacturing Research Conference, MSEC 2013
CountryUnited States
CityMadison, WI
Period6/10/136/14/13

Fingerprint

Fluidics
Nozzles
Cells
Tissue
Fabrication
Alginate
Cartilage
Cell death
Biomaterials
Hydrogels
Printing
Geometry

All Science Journal Classification (ASJC) codes

  • Industrial and Manufacturing Engineering

Cite this

Yu, Y., & Ozbolat, I. T. (2013). Bioprinting induced cell damage in cellular micro-fluidic channel fabrication. In ASME 2013 International Manufacturing Science and Engineering Conference Collocated with the 41st North American Manufacturing Research Conference, MSEC 2013 [MSEC2013-1081] (ASME 2013 International Manufacturing Science and Engineering Conference Collocated with the 41st North American Manufacturing Research Conference, MSEC 2013; Vol. 1). https://doi.org/10.1115/MSEC2013-1081
Yu, Yin ; Ozbolat, Ibrahim Tarik. / Bioprinting induced cell damage in cellular micro-fluidic channel fabrication. ASME 2013 International Manufacturing Science and Engineering Conference Collocated with the 41st North American Manufacturing Research Conference, MSEC 2013. 2013. (ASME 2013 International Manufacturing Science and Engineering Conference Collocated with the 41st North American Manufacturing Research Conference, MSEC 2013).
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Yu, Y & Ozbolat, IT 2013, Bioprinting induced cell damage in cellular micro-fluidic channel fabrication. in ASME 2013 International Manufacturing Science and Engineering Conference Collocated with the 41st North American Manufacturing Research Conference, MSEC 2013., MSEC2013-1081, ASME 2013 International Manufacturing Science and Engineering Conference Collocated with the 41st North American Manufacturing Research Conference, MSEC 2013, vol. 1, ASME 2013 International Manufacturing Science and Engineering Conference Collocated with the 41st North American Manufacturing Research Conference, MSEC 2013, Madison, WI, United States, 6/10/13. https://doi.org/10.1115/MSEC2013-1081

Bioprinting induced cell damage in cellular micro-fluidic channel fabrication. / Yu, Yin; Ozbolat, Ibrahim Tarik.

ASME 2013 International Manufacturing Science and Engineering Conference Collocated with the 41st North American Manufacturing Research Conference, MSEC 2013. 2013. MSEC2013-1081 (ASME 2013 International Manufacturing Science and Engineering Conference Collocated with the 41st North American Manufacturing Research Conference, MSEC 2013; Vol. 1).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Yu Y, Ozbolat IT. Bioprinting induced cell damage in cellular micro-fluidic channel fabrication. In ASME 2013 International Manufacturing Science and Engineering Conference Collocated with the 41st North American Manufacturing Research Conference, MSEC 2013. 2013. MSEC2013-1081. (ASME 2013 International Manufacturing Science and Engineering Conference Collocated with the 41st North American Manufacturing Research Conference, MSEC 2013). https://doi.org/10.1115/MSEC2013-1081