Bioreduction of nitrobenzene, natural organic matter, and hematite by Shewanella putrefaciens CN32

Fubo Luan, William D. Burgos, Li Xi E, Qi Zhou

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

We examined the reduction of nitrobenzene by Shewanella putrefaciens CN32 in the presence of natural organic matter (NOM) and hematite. Bioreduction experiments were conducted with combinations and varied concentrations of nitrobenzene, soil humic acid, Georgetown NOM, hematite, and CN32. Abiotic experiments were conducted to quantify nitrobenzene reduction by biogenic Fe(II) and by bioreduced NOMs. We show that S. putrefaciens CN32 can directly reduce nitrobenzene. Both NOMs enhanced nitrobenzene reduction and the degree of enhancement depended on properties of the NOMs (aromaticity, organic radical content). Hematite enhanced nitrobenzene reduction by indirect reaction with biogenic-Fe(II), however, enhancement was dependent on the availability of excess electron donor. Under electron donorlimiting conditions, reducing equivalents diverted to hematite werenot all transferred to nitrobenzene. In systems that contained both NOM and hematite we conclude that NOM-mediated reduction of nitrobenzene was more important than Fe(II)-mediated reduction.

Original languageEnglish (US)
Pages (from-to)184-190
Number of pages7
JournalEnvironmental Science and Technology
Volume44
Issue number1
DOIs
StatePublished - Jan 1 2010

Fingerprint

Biological materials
hematite
organic matter
electron
Humic Substances
humic acid
nitrobenzene
ferric oxide
Electrons
experiment
Experiments
Availability
Soils
soil

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry

Cite this

@article{ae4741c276a04ca990750a81d8b74467,
title = "Bioreduction of nitrobenzene, natural organic matter, and hematite by Shewanella putrefaciens CN32",
abstract = "We examined the reduction of nitrobenzene by Shewanella putrefaciens CN32 in the presence of natural organic matter (NOM) and hematite. Bioreduction experiments were conducted with combinations and varied concentrations of nitrobenzene, soil humic acid, Georgetown NOM, hematite, and CN32. Abiotic experiments were conducted to quantify nitrobenzene reduction by biogenic Fe(II) and by bioreduced NOMs. We show that S. putrefaciens CN32 can directly reduce nitrobenzene. Both NOMs enhanced nitrobenzene reduction and the degree of enhancement depended on properties of the NOMs (aromaticity, organic radical content). Hematite enhanced nitrobenzene reduction by indirect reaction with biogenic-Fe(II), however, enhancement was dependent on the availability of excess electron donor. Under electron donorlimiting conditions, reducing equivalents diverted to hematite werenot all transferred to nitrobenzene. In systems that contained both NOM and hematite we conclude that NOM-mediated reduction of nitrobenzene was more important than Fe(II)-mediated reduction.",
author = "Fubo Luan and Burgos, {William D.} and {Xi E}, Li and Qi Zhou",
year = "2010",
month = "1",
day = "1",
doi = "10.1021/es901585z",
language = "English (US)",
volume = "44",
pages = "184--190",
journal = "Environmental Science & Technology",
issn = "0013-936X",
publisher = "American Chemical Society",
number = "1",

}

Bioreduction of nitrobenzene, natural organic matter, and hematite by Shewanella putrefaciens CN32. / Luan, Fubo; Burgos, William D.; Xi E, Li; Zhou, Qi.

In: Environmental Science and Technology, Vol. 44, No. 1, 01.01.2010, p. 184-190.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Bioreduction of nitrobenzene, natural organic matter, and hematite by Shewanella putrefaciens CN32

AU - Luan, Fubo

AU - Burgos, William D.

AU - Xi E, Li

AU - Zhou, Qi

PY - 2010/1/1

Y1 - 2010/1/1

N2 - We examined the reduction of nitrobenzene by Shewanella putrefaciens CN32 in the presence of natural organic matter (NOM) and hematite. Bioreduction experiments were conducted with combinations and varied concentrations of nitrobenzene, soil humic acid, Georgetown NOM, hematite, and CN32. Abiotic experiments were conducted to quantify nitrobenzene reduction by biogenic Fe(II) and by bioreduced NOMs. We show that S. putrefaciens CN32 can directly reduce nitrobenzene. Both NOMs enhanced nitrobenzene reduction and the degree of enhancement depended on properties of the NOMs (aromaticity, organic radical content). Hematite enhanced nitrobenzene reduction by indirect reaction with biogenic-Fe(II), however, enhancement was dependent on the availability of excess electron donor. Under electron donorlimiting conditions, reducing equivalents diverted to hematite werenot all transferred to nitrobenzene. In systems that contained both NOM and hematite we conclude that NOM-mediated reduction of nitrobenzene was more important than Fe(II)-mediated reduction.

AB - We examined the reduction of nitrobenzene by Shewanella putrefaciens CN32 in the presence of natural organic matter (NOM) and hematite. Bioreduction experiments were conducted with combinations and varied concentrations of nitrobenzene, soil humic acid, Georgetown NOM, hematite, and CN32. Abiotic experiments were conducted to quantify nitrobenzene reduction by biogenic Fe(II) and by bioreduced NOMs. We show that S. putrefaciens CN32 can directly reduce nitrobenzene. Both NOMs enhanced nitrobenzene reduction and the degree of enhancement depended on properties of the NOMs (aromaticity, organic radical content). Hematite enhanced nitrobenzene reduction by indirect reaction with biogenic-Fe(II), however, enhancement was dependent on the availability of excess electron donor. Under electron donorlimiting conditions, reducing equivalents diverted to hematite werenot all transferred to nitrobenzene. In systems that contained both NOM and hematite we conclude that NOM-mediated reduction of nitrobenzene was more important than Fe(II)-mediated reduction.

UR - http://www.scopus.com/inward/record.url?scp=75349094071&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=75349094071&partnerID=8YFLogxK

U2 - 10.1021/es901585z

DO - 10.1021/es901585z

M3 - Article

VL - 44

SP - 184

EP - 190

JO - Environmental Science & Technology

JF - Environmental Science & Technology

SN - 0013-936X

IS - 1

ER -