Bioresponsive peptide-polysaccharide nanogels — A versatile delivery system to augment the utility of bioactive cargo

Andrew W. Simonson, Atip Lawanprasert, Tyler D.P. Goralski, Kenneth Charles Keiler, Scott H. Medina

Research output: Contribution to journalArticle

Abstract

We report the design, synthesis and efficacy of a new class of gel-like nano-carrier, or ‘nanogel’, prepared via templated electrostatic assembly of anionic hyaluronic acid (HA) polysaccharides with the cationic peptide amphiphile poly-L-lysine (PLL). Small molecules and proteins present during nanogel assembly become directly encapsulated within the carrier and are precisely released by tuning the nanogel HA:PLL ratio to control particle swelling. Remarkably, nanogels exhibit versatile and complimentary mechanisms of cargo delivery depending on the biologic context. For example, in mammalian cells, nanogels are rapidly internalized and escape the endosome to both deliver membrane-impermeable protein cargo into the cytoplasm and improve chemotherapeutic potency in drug resistant cancer cells. In bacteria, nanogels permeabilize microbial membranes to sensitize bacterial pathogens to the action of a loaded antibiotic. Thus, peptide nanogels represent a versatile, readily scalable and bio-responsive carrier capable of augmenting and enhancing the utility of a broad range of biomolecular cargoes.

Original languageEnglish (US)
Pages (from-to)391-400
Number of pages10
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume17
DOIs
StatePublished - Apr 1 2019

Fingerprint

Hyaluronic acid
Polysaccharides
Peptides
Cells
Proteins
Membranes
Amphiphiles
Pathogens
Antibiotics
Swelling
Electrostatics
Bacteria
Gels
Tuning
Molecules
Hyaluronic Acid
Lysine
Endosomes
NanoGel
polysaccharide peptide

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

Cite this

@article{cd3b8516202040e793c8d9d2b320adda,
title = "Bioresponsive peptide-polysaccharide nanogels — A versatile delivery system to augment the utility of bioactive cargo",
abstract = "We report the design, synthesis and efficacy of a new class of gel-like nano-carrier, or ‘nanogel’, prepared via templated electrostatic assembly of anionic hyaluronic acid (HA) polysaccharides with the cationic peptide amphiphile poly-L-lysine (PLL). Small molecules and proteins present during nanogel assembly become directly encapsulated within the carrier and are precisely released by tuning the nanogel HA:PLL ratio to control particle swelling. Remarkably, nanogels exhibit versatile and complimentary mechanisms of cargo delivery depending on the biologic context. For example, in mammalian cells, nanogels are rapidly internalized and escape the endosome to both deliver membrane-impermeable protein cargo into the cytoplasm and improve chemotherapeutic potency in drug resistant cancer cells. In bacteria, nanogels permeabilize microbial membranes to sensitize bacterial pathogens to the action of a loaded antibiotic. Thus, peptide nanogels represent a versatile, readily scalable and bio-responsive carrier capable of augmenting and enhancing the utility of a broad range of biomolecular cargoes.",
author = "Simonson, {Andrew W.} and Atip Lawanprasert and Goralski, {Tyler D.P.} and Keiler, {Kenneth Charles} and Medina, {Scott H.}",
year = "2019",
month = "4",
day = "1",
doi = "10.1016/j.nano.2018.10.008",
language = "English (US)",
volume = "17",
pages = "391--400",
journal = "Nanomedicine: Nanotechnology, Biology, and Medicine",
issn = "1549-9634",
publisher = "Elsevier Inc.",

}

Bioresponsive peptide-polysaccharide nanogels — A versatile delivery system to augment the utility of bioactive cargo. / Simonson, Andrew W.; Lawanprasert, Atip; Goralski, Tyler D.P.; Keiler, Kenneth Charles; Medina, Scott H.

In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 17, 01.04.2019, p. 391-400.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Bioresponsive peptide-polysaccharide nanogels — A versatile delivery system to augment the utility of bioactive cargo

AU - Simonson, Andrew W.

AU - Lawanprasert, Atip

AU - Goralski, Tyler D.P.

AU - Keiler, Kenneth Charles

AU - Medina, Scott H.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - We report the design, synthesis and efficacy of a new class of gel-like nano-carrier, or ‘nanogel’, prepared via templated electrostatic assembly of anionic hyaluronic acid (HA) polysaccharides with the cationic peptide amphiphile poly-L-lysine (PLL). Small molecules and proteins present during nanogel assembly become directly encapsulated within the carrier and are precisely released by tuning the nanogel HA:PLL ratio to control particle swelling. Remarkably, nanogels exhibit versatile and complimentary mechanisms of cargo delivery depending on the biologic context. For example, in mammalian cells, nanogels are rapidly internalized and escape the endosome to both deliver membrane-impermeable protein cargo into the cytoplasm and improve chemotherapeutic potency in drug resistant cancer cells. In bacteria, nanogels permeabilize microbial membranes to sensitize bacterial pathogens to the action of a loaded antibiotic. Thus, peptide nanogels represent a versatile, readily scalable and bio-responsive carrier capable of augmenting and enhancing the utility of a broad range of biomolecular cargoes.

AB - We report the design, synthesis and efficacy of a new class of gel-like nano-carrier, or ‘nanogel’, prepared via templated electrostatic assembly of anionic hyaluronic acid (HA) polysaccharides with the cationic peptide amphiphile poly-L-lysine (PLL). Small molecules and proteins present during nanogel assembly become directly encapsulated within the carrier and are precisely released by tuning the nanogel HA:PLL ratio to control particle swelling. Remarkably, nanogels exhibit versatile and complimentary mechanisms of cargo delivery depending on the biologic context. For example, in mammalian cells, nanogels are rapidly internalized and escape the endosome to both deliver membrane-impermeable protein cargo into the cytoplasm and improve chemotherapeutic potency in drug resistant cancer cells. In bacteria, nanogels permeabilize microbial membranes to sensitize bacterial pathogens to the action of a loaded antibiotic. Thus, peptide nanogels represent a versatile, readily scalable and bio-responsive carrier capable of augmenting and enhancing the utility of a broad range of biomolecular cargoes.

UR - http://www.scopus.com/inward/record.url?scp=85057065203&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85057065203&partnerID=8YFLogxK

U2 - 10.1016/j.nano.2018.10.008

DO - 10.1016/j.nano.2018.10.008

M3 - Article

C2 - 30399437

AN - SCOPUS:85057065203

VL - 17

SP - 391

EP - 400

JO - Nanomedicine: Nanotechnology, Biology, and Medicine

JF - Nanomedicine: Nanotechnology, Biology, and Medicine

SN - 1549-9634

ER -