Biosynthesis of cellulose binding domains (CBDs)

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cellulose binding domains (CBDs) are discrete protein modules found in most cellulolytic enzymes which are distinct from catalytic domains. The presence of a CBD improves the binding and facilitates the activity of the catalytic domain on the insoluble cellulose substrates. The objectives of this study were to synthesize cellulose binding domains for the later research on understanding the interaction between CBDs and cellulose and cellulose-based materials, as well as to generate CBD-integrated nanocomposites for cellulose fiber organization engineering. Fungal CBDs from Trichoderma reesei cellobiohydrolase 1 (CBH1) and cellobiohydrolase 2 (CBH2) were heterologously expressed in host strain Escherichia coli BL21 (DE3) by constructing CSD-pET31b expression recombinant. The CBD coding sequences were cloned downstream of a 125 amino acid ketosteroid isomerase (KSI) gene and upstream of a hexa-histidine tag sequence in pET31b(+) vector and cleaved by CNBr to release target CBD peptides. The expressed proteins were purified by Ni-NTA immobilized metal affinity chromatography (IMAC). The application of this expression approach represents a fast and efficient method to prepare cellulose binding domain in its biologically active form, which makes it possible to further create the cellulose binding domain-integrated molecular linker to engineer the organization of cellulose-base materials and to create new functional cellulose nanocomposites.

Original languageEnglish (US)
Title of host publicationAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2009, ASABE 2009
Pages5075-5087
Number of pages13
StatePublished - Dec 1 2009
EventAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2009 - Reno, NV, United States
Duration: Jun 21 2009Jun 24 2009

Publication series

NameAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2009, ASABE 2009
Volume8

Other

OtherAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2009
CountryUnited States
CityReno, NV
Period6/21/096/24/09

Fingerprint

Cellulose
cellulose
biosynthesis
cellulose 1,4-beta-cellobiosidase
Cellulose 1,4-beta-Cellobiosidase
nanocomposites
Nanocomposites
active sites
Amino Acid Isomerases
ketosteroids
Catalytic Domain
Ketosteroids
host strains
cellulosic fibers
Trichoderma reesei
Trichoderma
isomerases
affinity chromatography
histidine
engineers

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

Cite this

Guo, J., Tien, M., & Catchmark, J. M. (2009). Biosynthesis of cellulose binding domains (CBDs). In American Society of Agricultural and Biological Engineers Annual International Meeting 2009, ASABE 2009 (pp. 5075-5087). (American Society of Agricultural and Biological Engineers Annual International Meeting 2009, ASABE 2009; Vol. 8).
Guo, Jing ; Tien, Ming ; Catchmark, Jeffrey M. / Biosynthesis of cellulose binding domains (CBDs). American Society of Agricultural and Biological Engineers Annual International Meeting 2009, ASABE 2009. 2009. pp. 5075-5087 (American Society of Agricultural and Biological Engineers Annual International Meeting 2009, ASABE 2009).
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Guo, J, Tien, M & Catchmark, JM 2009, Biosynthesis of cellulose binding domains (CBDs). in American Society of Agricultural and Biological Engineers Annual International Meeting 2009, ASABE 2009. American Society of Agricultural and Biological Engineers Annual International Meeting 2009, ASABE 2009, vol. 8, pp. 5075-5087, American Society of Agricultural and Biological Engineers Annual International Meeting 2009, Reno, NV, United States, 6/21/09.

Biosynthesis of cellulose binding domains (CBDs). / Guo, Jing; Tien, Ming; Catchmark, Jeffrey M.

American Society of Agricultural and Biological Engineers Annual International Meeting 2009, ASABE 2009. 2009. p. 5075-5087 (American Society of Agricultural and Biological Engineers Annual International Meeting 2009, ASABE 2009; Vol. 8).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Guo J, Tien M, Catchmark JM. Biosynthesis of cellulose binding domains (CBDs). In American Society of Agricultural and Biological Engineers Annual International Meeting 2009, ASABE 2009. 2009. p. 5075-5087. (American Society of Agricultural and Biological Engineers Annual International Meeting 2009, ASABE 2009).