Biosynthesis of Mammary Glycoproteins: Structural Characterization of Lipid‐Linked Glucosyloligosaccharides

Inder K. VIJAY, Gary H. PERDEW

Research output: Contribution to journalArticle

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Abstract

The lipid‐linked glucosyloligosaccharide intermediates for the biosynthesis of asparagine‐linked glycoproteins in the lactating bovine mammary tissue were isolated from incubations of microsomes of this tissue with labeled sugar‐nucleotides. These were shown to have the general structure (Glc)1–3(Man)9(GlcNAc)2. A structural characterization indicated that glucose residues in these oligosaccharides are linked as Glcα1 → 3, Glcα1 → 3Glcα1 → 3, and Glc1 → 2Glcα1 → 3Glcα1 → 3, to the tetramannosyl branch of (Man)9(GlcNAc)2 portion previously characterized as Manα1 → 2Manα1 → 2Manα1 → 3(Manα1 → 2Manα1 → 6[Manα1 → 2Manα1 → 3]‐Manα1 → 6)Manβ1 → 4(3)GlcNAcβ1 → 4(3)GlcNAc [Vijay, I. K., Perdew, G. H., and Lewis, D. E. (1980) J. Biol. Chem. 255, 11210–11220]. The lipid‐linked (Man)9(GlcNAc)2 appears to be the initial acceptor of the first glucosyl residue. Even though incubations of the bovine mammary tissue in vitro synthesize multiple isomers of a number of mannosyloligosaccharides, the glucosyloligosaccharides are all monoisomeric.

Original languageEnglish (US)
Pages (from-to)167-172
Number of pages6
JournalEuropean Journal of Biochemistry
Volume126
Issue number1
DOIs
StatePublished - Aug 1982

Fingerprint

Biosynthesis
Breast
Tissue
Microsomes
Oligosaccharides
Isomers
Glycoproteins
Glucose
mannosyl(9)-N-acetylglucosamine(2)
Bos taurus structural-GP protein

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

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abstract = "The lipid‐linked glucosyloligosaccharide intermediates for the biosynthesis of asparagine‐linked glycoproteins in the lactating bovine mammary tissue were isolated from incubations of microsomes of this tissue with labeled sugar‐nucleotides. These were shown to have the general structure (Glc)1–3(Man)9(GlcNAc)2. A structural characterization indicated that glucose residues in these oligosaccharides are linked as Glcα1 → 3, Glcα1 → 3Glcα1 → 3, and Glc1 → 2Glcα1 → 3Glcα1 → 3, to the tetramannosyl branch of (Man)9(GlcNAc)2 portion previously characterized as Manα1 → 2Manα1 → 2Manα1 → 3(Manα1 → 2Manα1 → 6[Manα1 → 2Manα1 → 3]‐Manα1 → 6)Manβ1 → 4(3)GlcNAcβ1 → 4(3)GlcNAc [Vijay, I. K., Perdew, G. H., and Lewis, D. E. (1980) J. Biol. Chem. 255, 11210–11220]. The lipid‐linked (Man)9(GlcNAc)2 appears to be the initial acceptor of the first glucosyl residue. Even though incubations of the bovine mammary tissue in vitro synthesize multiple isomers of a number of mannosyloligosaccharides, the glucosyloligosaccharides are all monoisomeric.",
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Biosynthesis of Mammary Glycoproteins : Structural Characterization of Lipid‐Linked Glucosyloligosaccharides. / VIJAY, Inder K.; PERDEW, Gary H.

In: European Journal of Biochemistry, Vol. 126, No. 1, 08.1982, p. 167-172.

Research output: Contribution to journalArticle

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