Biotechnology has been practiced on chestnuts (Castanea spp.) for many decades, including vegetative propagation, controlled crossing followed by testing and selection, genetic and cytogenetic mapping, genetic modifi cation, and gene and genome sequencing. Vegetative propagation methods have ranged from grafting and rooting to somatic embryogenesis, often in coordination with breeding efforts and programs. More recently, particularly in the United States, chestnut biotechnology has included the analysis of genes and genomes with the goal of characterizing and fi nding disease resistance genes and utilizing them for developing resistant Castanea dentata (American chestnut) for use in species conservation programs. In this chapter we review chestnut biotechnology, especially with respect to its development in the United States, with emphasis on producing resistant C. dentata using various resistant sources of C. mollissma (Chinese chestnut) and C. crenata (Japanese chestnut).
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)