Blocking free radical production via adenoviral gene transfer decreases cardiac ischemia-reperfusion injury

Henry L. Zhu, Allan S. Stewart, Matthew D. Taylor, C. Vijayasarathy, Timothy J. Gardner, H. Lee Sweeney

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Periods of cardiac ischemia followed by reperfusion can lead to either transient loss of function (stunning) or permanent functional loss stemming from infarction, depending upon the length of the ischemic period. In either case the primary mediator of the injury may by oxygen-derived free radicals generated upon the reestablishment of blood flow. The heart's primary defense against peroxide, glutathione peroxidase, is depleted during ischemia. Thus, the ischemic myocardium might derive significant protection from increased levels of the enzyme, catalase, which can remove hydrogen peroxide in a redox-independent manner. To test these assertions, we studied the ability of adenoviral gene transfer to increase intracellular antioxidant activity via catalase expression. What we observed was that increasing catalase activity in the heart was sufficient to prevent the stunning associated with 15 min of ischemia followed by reperfusion.

Original languageEnglish (US)
Pages (from-to)470-475
Number of pages6
JournalMolecular Therapy
Volume2
Issue number5
DOIs
StatePublished - Nov 2000

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Pharmacology
  • Drug Discovery

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