Blood glutathione concentrations in a large-scale human study

John Richie, Lisa Skowronski, Patricia Abraham, Yvonne Leutzinger

Research output: Contribution to journalArticle

188 Citations (Scopus)

Abstract

Little is known about the variability of blood glutathione (GSII) in human subjects. Thus, we wanted to develop and validate a rapid method for measuring GSH concentrations in whole blood and apply this method to ascertain the variation of GSH in a large-scale study of free-living adults. The assay was highly sensitive (detection limit <5 pmol) and precise, with an interassay variation of 2.3% and a sampling variation of 3.6%. Applying this method to screen 715 adults, we observed a threefold range of GSH concentrations, with a mean of 1.02 mmol/L and CV of 17%. GSH concentrations were 8-10% greater in smokers than in nonsmokers (P <0.001). Although we observed no sex differences for GSH, GSH/hemoglobin ratios were 8-18% greater in women <55 years old than in older subjects (P <0.05). The results presented here validate the use of this method for large-scale human studies and provide information on the variation and normal values of blood GSH in adults.

Original languageEnglish (US)
Pages (from-to)64-70
Number of pages7
JournalClinical Chemistry
Volume42
Issue number1
StatePublished - Feb 2 1996

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Glutathione
Blood
Assays
Hemoglobins
Sex Characteristics
Sampling
Limit of Detection
Reference Values

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Richie, J., Skowronski, L., Abraham, P., & Leutzinger, Y. (1996). Blood glutathione concentrations in a large-scale human study. Clinical Chemistry, 42(1), 64-70.
Richie, John ; Skowronski, Lisa ; Abraham, Patricia ; Leutzinger, Yvonne. / Blood glutathione concentrations in a large-scale human study. In: Clinical Chemistry. 1996 ; Vol. 42, No. 1. pp. 64-70.
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Richie, J, Skowronski, L, Abraham, P & Leutzinger, Y 1996, 'Blood glutathione concentrations in a large-scale human study', Clinical Chemistry, vol. 42, no. 1, pp. 64-70.

Blood glutathione concentrations in a large-scale human study. / Richie, John; Skowronski, Lisa; Abraham, Patricia; Leutzinger, Yvonne.

In: Clinical Chemistry, Vol. 42, No. 1, 02.02.1996, p. 64-70.

Research output: Contribution to journalArticle

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Richie J, Skowronski L, Abraham P, Leutzinger Y. Blood glutathione concentrations in a large-scale human study. Clinical Chemistry. 1996 Feb 2;42(1):64-70.