Blood stage Plasmodium falciparum exhibits biological responses to direct current electric fields

Lorena M. Coronado, Stephania Montealegre, Zumara Chaverra, Luis Mojica, Carlos Espinosa, Alejandro Almanza, Ricardo Correa, Jose Stoute, Rolando A. Gittens, Carmenza Spadafora

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The development of resistance to insecticides by the vector of malaria and the increasingly faster appearance of resistance to antimalarial drugs by the parasite can dangerously hamper efforts to control and eradicate the disease. Alternative ways to treat this disease are urgently needed. Here we evaluate the in vitro effect of direct current (DC) capacitive coupling electrical stimulation on the biology and viability of Plasmodium falciparum. We designed a system that exposes infected erythrocytes to different capacitively coupled electric fields in order to evaluate their effect on P. falciparum. The effect on growth of the parasite, replication of DNA, mitochondrial membrane potential and level of reactive oxygen species after exposure to electric fields demonstrate that the parasite is biologically able to respond to stimuli from DC electric fields involving calcium signaling pathways.

Original languageEnglish (US)
Article numbere0161207
JournalPloS one
Volume11
Issue number8
DOIs
StatePublished - Aug 1 2016

Fingerprint

Plasmodium falciparum
electric field
Parasites
Blood
Electric fields
parasites
blood
Insecticide Resistance
antimalarials
Calcium Signaling
Mitochondrial Membrane Potential
Antimalarials
DNA replication
Insecticides
DNA Replication
Mitochondrial DNA
membrane potential
malaria
Electric Stimulation
Malaria

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Coronado, L. M., Montealegre, S., Chaverra, Z., Mojica, L., Espinosa, C., Almanza, A., ... Spadafora, C. (2016). Blood stage Plasmodium falciparum exhibits biological responses to direct current electric fields. PloS one, 11(8), [e0161207]. https://doi.org/10.1371/journal.pone.0161207
Coronado, Lorena M. ; Montealegre, Stephania ; Chaverra, Zumara ; Mojica, Luis ; Espinosa, Carlos ; Almanza, Alejandro ; Correa, Ricardo ; Stoute, Jose ; Gittens, Rolando A. ; Spadafora, Carmenza. / Blood stage Plasmodium falciparum exhibits biological responses to direct current electric fields. In: PloS one. 2016 ; Vol. 11, No. 8.
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Coronado, LM, Montealegre, S, Chaverra, Z, Mojica, L, Espinosa, C, Almanza, A, Correa, R, Stoute, J, Gittens, RA & Spadafora, C 2016, 'Blood stage Plasmodium falciparum exhibits biological responses to direct current electric fields', PloS one, vol. 11, no. 8, e0161207. https://doi.org/10.1371/journal.pone.0161207

Blood stage Plasmodium falciparum exhibits biological responses to direct current electric fields. / Coronado, Lorena M.; Montealegre, Stephania; Chaverra, Zumara; Mojica, Luis; Espinosa, Carlos; Almanza, Alejandro; Correa, Ricardo; Stoute, Jose; Gittens, Rolando A.; Spadafora, Carmenza.

In: PloS one, Vol. 11, No. 8, e0161207, 01.08.2016.

Research output: Contribution to journalArticle

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Coronado LM, Montealegre S, Chaverra Z, Mojica L, Espinosa C, Almanza A et al. Blood stage Plasmodium falciparum exhibits biological responses to direct current electric fields. PloS one. 2016 Aug 1;11(8). e0161207. https://doi.org/10.1371/journal.pone.0161207