Border control - A membrane-linked interactome of Arabidopsis

Alexander M. Jones, Yuanhu Xuan, Meng Xu, Rui Sheng Wang, Cheng Hsun Ho, Sylvie Lalonde, Chang Hun You, Maria I. Sardi, Saman A. Parsa, Erika Smith-Valle, Tianying Su, Keith A. Frazer, Guillaume Pilot, Réjane Pratelli, Guido Grossmann, Biswa R. Acharya, Heng Cheng Hu, Cawas Engineer, Florent Villiers, Chuanli JuKouji Takeda, Zhao Su, Qunfeng Dong, Sarah M. Assmann, Jin Chen, June M. Kwak, Julian I. Schroeder, Reka Albert, Seung Y. Rhee, Wolf B. Frommer

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Cellular membranes act as signaling platforms and control solute transport. Membrane receptors, transporters, and enzymes communicate with intracellular processes through protein-protein interactions. Using a split-ubiquitin yeast two-hybrid screen that covers a test-space of 6.4 × 106 pairs, we identified 12,102 membrane/signaling protein interactions from Arabidopsis. Besides confirmation of expected interactions such as heterotrimeric G protein subunit interactions and aquaporin oligomerization, >99% of the interactions were previously unknown. Interactions were confirmed at a rate of 32% in orthogonal in planta split-green flourescent protein interaction assays, which was statistically indistinguishable from the confirmation rate for known interactions collected from literature (38%). Regulatory associations in membrane protein trafficking, turnover, and phosphorylation include regulation of potassium channel activity through abscisic acid signaling, transporter activity by a WNK kinase, and a brassinolide receptor kinase by trafficking-related proteins. These examples underscore the utility of the membrane/signaling protein interaction network for gene discovery and hypothesis generation in plants and other organisms.

Original languageEnglish (US)
Pages (from-to)711-716
Number of pages6
JournalScience
Volume344
Issue number6185
DOIs
StatePublished - Jan 1 2014

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Arabidopsis
Membrane Proteins
Protein Transport
Membranes
Phosphotransferases
Protein Interaction Maps
Heterotrimeric GTP-Binding Proteins
Abscisic Acid
Aquaporins
Proteins
Membrane Transport Proteins
Potassium Channels
Protein Subunits
Genetic Association Studies
Ubiquitin
Yeasts
Phosphorylation
Enzymes

All Science Journal Classification (ASJC) codes

  • General

Cite this

Jones, A. M., Xuan, Y., Xu, M., Wang, R. S., Ho, C. H., Lalonde, S., ... Frommer, W. B. (2014). Border control - A membrane-linked interactome of Arabidopsis. Science, 344(6185), 711-716. https://doi.org/10.1126/science.1251358
Jones, Alexander M. ; Xuan, Yuanhu ; Xu, Meng ; Wang, Rui Sheng ; Ho, Cheng Hsun ; Lalonde, Sylvie ; You, Chang Hun ; Sardi, Maria I. ; Parsa, Saman A. ; Smith-Valle, Erika ; Su, Tianying ; Frazer, Keith A. ; Pilot, Guillaume ; Pratelli, Réjane ; Grossmann, Guido ; Acharya, Biswa R. ; Hu, Heng Cheng ; Engineer, Cawas ; Villiers, Florent ; Ju, Chuanli ; Takeda, Kouji ; Su, Zhao ; Dong, Qunfeng ; Assmann, Sarah M. ; Chen, Jin ; Kwak, June M. ; Schroeder, Julian I. ; Albert, Reka ; Rhee, Seung Y. ; Frommer, Wolf B. / Border control - A membrane-linked interactome of Arabidopsis. In: Science. 2014 ; Vol. 344, No. 6185. pp. 711-716.
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Jones, AM, Xuan, Y, Xu, M, Wang, RS, Ho, CH, Lalonde, S, You, CH, Sardi, MI, Parsa, SA, Smith-Valle, E, Su, T, Frazer, KA, Pilot, G, Pratelli, R, Grossmann, G, Acharya, BR, Hu, HC, Engineer, C, Villiers, F, Ju, C, Takeda, K, Su, Z, Dong, Q, Assmann, SM, Chen, J, Kwak, JM, Schroeder, JI, Albert, R, Rhee, SY & Frommer, WB 2014, 'Border control - A membrane-linked interactome of Arabidopsis', Science, vol. 344, no. 6185, pp. 711-716. https://doi.org/10.1126/science.1251358

Border control - A membrane-linked interactome of Arabidopsis. / Jones, Alexander M.; Xuan, Yuanhu; Xu, Meng; Wang, Rui Sheng; Ho, Cheng Hsun; Lalonde, Sylvie; You, Chang Hun; Sardi, Maria I.; Parsa, Saman A.; Smith-Valle, Erika; Su, Tianying; Frazer, Keith A.; Pilot, Guillaume; Pratelli, Réjane; Grossmann, Guido; Acharya, Biswa R.; Hu, Heng Cheng; Engineer, Cawas; Villiers, Florent; Ju, Chuanli; Takeda, Kouji; Su, Zhao; Dong, Qunfeng; Assmann, Sarah M.; Chen, Jin; Kwak, June M.; Schroeder, Julian I.; Albert, Reka; Rhee, Seung Y.; Frommer, Wolf B.

In: Science, Vol. 344, No. 6185, 01.01.2014, p. 711-716.

Research output: Contribution to journalArticle

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AU - You, Chang Hun

AU - Sardi, Maria I.

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AU - Frazer, Keith A.

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AU - Grossmann, Guido

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AU - Ju, Chuanli

AU - Takeda, Kouji

AU - Su, Zhao

AU - Dong, Qunfeng

AU - Assmann, Sarah M.

AU - Chen, Jin

AU - Kwak, June M.

AU - Schroeder, Julian I.

AU - Albert, Reka

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Jones AM, Xuan Y, Xu M, Wang RS, Ho CH, Lalonde S et al. Border control - A membrane-linked interactome of Arabidopsis. Science. 2014 Jan 1;344(6185):711-716. https://doi.org/10.1126/science.1251358