Bordetella bronchiseptica PagP is a Bvg-regulated lipid A palmitoyl transferase that is required for persistent colonization of the mouse respiratory tract

Andrew Preston, Elizabeth Maxim, Elinor Toland, E. Jane Pishko, Eric Thomas Harvill, Martine Caroff, Duncan J. Maskell

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Bordetella bronchiseptica lipopolysaccharide (LPS) expression varies depending on growth conditions, regulated by the Bvg system. A B. bronchiseptica pagP homologue was identified that is required for Bvg-mediated modification of the lipid A core region of LPS that occurs on switching from the Bvg- to the Bvg+ phase. Structural analysis demonstrated that the lipid A of a B. bronchiseptica pagP mutant differed from wild-type lipid A by the absence of a palmitate group in secondary acylation at the C3′ position. The putative pagP promoter drove the expression of a green fluorescent protein (GFP) reporter gene in a Bvg-regulated fashion. These data suggest that B. bronchiseptica pagP encodes a Bvg-regulated lipid A palmitoyl transferase that mediates modification of the lipid A as part of the overall Bvg-mediated adaptation of this organism to changing environmental conditions. We also show that pagP is not required for the initial colonization of the mouse respiratory tract by B. bronchiseptica, but is required for persistence of the organism within this organ.

Original languageEnglish (US)
Pages (from-to)725-736
Number of pages12
JournalMolecular Microbiology
Volume48
Issue number3
DOIs
StatePublished - May 1 2003

Fingerprint

Bordetella bronchiseptica
Lipid A
Transferases
Respiratory System
Lipopolysaccharides
Acylation
Palmitates
Green Fluorescent Proteins
Reporter Genes
Growth

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Preston, Andrew ; Maxim, Elizabeth ; Toland, Elinor ; Pishko, E. Jane ; Harvill, Eric Thomas ; Caroff, Martine ; Maskell, Duncan J. / Bordetella bronchiseptica PagP is a Bvg-regulated lipid A palmitoyl transferase that is required for persistent colonization of the mouse respiratory tract. In: Molecular Microbiology. 2003 ; Vol. 48, No. 3. pp. 725-736.
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Bordetella bronchiseptica PagP is a Bvg-regulated lipid A palmitoyl transferase that is required for persistent colonization of the mouse respiratory tract. / Preston, Andrew; Maxim, Elizabeth; Toland, Elinor; Pishko, E. Jane; Harvill, Eric Thomas; Caroff, Martine; Maskell, Duncan J.

In: Molecular Microbiology, Vol. 48, No. 3, 01.05.2003, p. 725-736.

Research output: Contribution to journalArticle

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