Bordetella parapertussis survives inside human macrophages in lipid raft-enriched phagosomes

Juan Gorgojo, Eric Thomas Harvill, Maria Eugenia Rodríguez

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Bordetella parapertussis is a human pathogen that causes whooping cough. The increasing incidence of B. parapertussis has been attributed to the lack of cross protection induced by pertussis vaccines. It was previously shown that B. parapertussis is able to avoid bacterial killing by polymorphonuclear leukocytes (PMN) if specific opsonic antibodies are not present at the site of interaction. Here, we evaluated the outcome of B. parapertussis innate interaction with human macrophages, a less aggressive type of cell and a known reservoir of many persistent pathogens. The results showed that in the absence of opsonins, O antigen allows B. parapertussis to inhibit phagolysosomal fusion and to remain alive inside macrophages. The O antigen targets B. parapertussis to lipid rafts that are retained in the membrane of phagosomes that do not undergo lysosomal maturation. Fortyeight hours after infection, wild-type B. parapertussis bacteria but not the O antigen-deficient mutants were found colocalizing with lipid rafts and alive in nonacidic compartments. Taken together, our data suggest that in the absence of opsonic antibodies, B. parapertussis survives inside macrophages by preventing phagolysosomal maturation in a lipid raft- and O antigen-dependent manner. Two days after infection, about 15% of macrophages were found loaded with live bacteria inside flotillin-enriched phagosomes that had access to nutrients provided by the host cell recycling pathway, suggesting the development of an intracellular infection. IgG opsonization drastically changed this interaction, inducing efficient bacterial killing. These results highlight the need for B. parapertussis opsonic antibodies to induce bacterial clearance and prevent the eventual establishment of cellular reservoirs of this pathogen.

Original languageEnglish (US)
Pages (from-to)5175-5184
Number of pages10
JournalInfection and Immunity
Volume82
Issue number12
DOIs
StatePublished - Jan 1 2014

Fingerprint

Bordetella parapertussis
Phagosomes
Macrophages
Lipids
O Antigens
Antibodies
Infection
Opsonin Proteins
Cross Protection
Bacteria
Pertussis Vaccine
Whooping Cough
Neutrophils
Immunoglobulin G

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Gorgojo, Juan ; Harvill, Eric Thomas ; Rodríguez, Maria Eugenia. / Bordetella parapertussis survives inside human macrophages in lipid raft-enriched phagosomes. In: Infection and Immunity. 2014 ; Vol. 82, No. 12. pp. 5175-5184.
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Bordetella parapertussis survives inside human macrophages in lipid raft-enriched phagosomes. / Gorgojo, Juan; Harvill, Eric Thomas; Rodríguez, Maria Eugenia.

In: Infection and Immunity, Vol. 82, No. 12, 01.01.2014, p. 5175-5184.

Research output: Contribution to journalArticle

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AU - Harvill, Eric Thomas

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