Bordetella pseudohinzii as a confounding organism in murine models of pulmonary disease

Sarah Clark, Jeanette E. Purcell, Saad Sammani, Earl K. Steffen, Marcus J. Crim, Robert S. Livingston, Cynthia Besch-Williford, Jeffrey D. Fortman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A group studying acute lung injury observed an increased percentage of neutrophils in the bronchoalveolar lavage (BAL) fluid of mice. BAL was performed, and lung samples were collected sterilely from 5 C57BL/6 mice that had been bred inhouse. Pure colonies of bacteria, initially identified as Bordetella hinzii were cultured from 2 of the 5 mice which had the highest percentages of neutrophils (21% and 26%) in the BAL fluid. Subsequent sequencing of a portion of the ompA gene from this isolate demonstrated 100% homology with the published B. pseudohinzii sequence. We then selected 10 mice from the investigator's colony to determine the best test to screen for B. pseudohinzii in the facility. BAL was performed, the left lung lobe was collected for culture and PCR analysis, the right lung lobe and nasal passages were collected for histopathology, an oral swab was collected for culture, and an oral swab and fecal pellets were collected for PCR analysis. B. pseudohinzii was cultured from the oral cavity, lung, or both in 8 of the 10 mice analyzed. All 8 of these mice were fecal PCR positive for B. pseudohinzii; 7 had increased neutrophils (5% to 20%) in the BAL fluid, whereas the 8th mouse had a normal neutrophil percentage (2%). Active bronchopneumonia was not observed, but some infected mice had mild to moderate rhinitis. B. pseudohinzii appears to be a microbial agent of importance in mouse colonies that can confound pulmonary research. Commercial vendors and institutions should consider colony screening, routine reporting, and exclusion of B. pseudohinzii.

Original languageEnglish (US)
Pages (from-to)361-366
Number of pages6
JournalComparative Medicine
Volume66
Issue number5
StatePublished - Oct 1 2016

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Bordetella
Pulmonary diseases
respiratory tract diseases
Lung Diseases
animal models
Fluids
organisms
mice
lungs
Bronchoalveolar Lavage Fluid
Neutrophils
Lung
neutrophils
mouth
Bacteria
Screening
Bronchoalveolar Lavage
Genes
Polymerase Chain Reaction
Bordetella hinzii

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

Cite this

Clark, S., Purcell, J. E., Sammani, S., Steffen, E. K., Crim, M. J., Livingston, R. S., ... Fortman, J. D. (2016). Bordetella pseudohinzii as a confounding organism in murine models of pulmonary disease. Comparative Medicine, 66(5), 361-366.
Clark, Sarah ; Purcell, Jeanette E. ; Sammani, Saad ; Steffen, Earl K. ; Crim, Marcus J. ; Livingston, Robert S. ; Besch-Williford, Cynthia ; Fortman, Jeffrey D. / Bordetella pseudohinzii as a confounding organism in murine models of pulmonary disease. In: Comparative Medicine. 2016 ; Vol. 66, No. 5. pp. 361-366.
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abstract = "A group studying acute lung injury observed an increased percentage of neutrophils in the bronchoalveolar lavage (BAL) fluid of mice. BAL was performed, and lung samples were collected sterilely from 5 C57BL/6 mice that had been bred inhouse. Pure colonies of bacteria, initially identified as Bordetella hinzii were cultured from 2 of the 5 mice which had the highest percentages of neutrophils (21{\%} and 26{\%}) in the BAL fluid. Subsequent sequencing of a portion of the ompA gene from this isolate demonstrated 100{\%} homology with the published B. pseudohinzii sequence. We then selected 10 mice from the investigator's colony to determine the best test to screen for B. pseudohinzii in the facility. BAL was performed, the left lung lobe was collected for culture and PCR analysis, the right lung lobe and nasal passages were collected for histopathology, an oral swab was collected for culture, and an oral swab and fecal pellets were collected for PCR analysis. B. pseudohinzii was cultured from the oral cavity, lung, or both in 8 of the 10 mice analyzed. All 8 of these mice were fecal PCR positive for B. pseudohinzii; 7 had increased neutrophils (5{\%} to 20{\%}) in the BAL fluid, whereas the 8th mouse had a normal neutrophil percentage (2{\%}). Active bronchopneumonia was not observed, but some infected mice had mild to moderate rhinitis. B. pseudohinzii appears to be a microbial agent of importance in mouse colonies that can confound pulmonary research. Commercial vendors and institutions should consider colony screening, routine reporting, and exclusion of B. pseudohinzii.",
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Clark, S, Purcell, JE, Sammani, S, Steffen, EK, Crim, MJ, Livingston, RS, Besch-Williford, C & Fortman, JD 2016, 'Bordetella pseudohinzii as a confounding organism in murine models of pulmonary disease', Comparative Medicine, vol. 66, no. 5, pp. 361-366.

Bordetella pseudohinzii as a confounding organism in murine models of pulmonary disease. / Clark, Sarah; Purcell, Jeanette E.; Sammani, Saad; Steffen, Earl K.; Crim, Marcus J.; Livingston, Robert S.; Besch-Williford, Cynthia; Fortman, Jeffrey D.

In: Comparative Medicine, Vol. 66, No. 5, 01.10.2016, p. 361-366.

Research output: Contribution to journalArticle

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Clark S, Purcell JE, Sammani S, Steffen EK, Crim MJ, Livingston RS et al. Bordetella pseudohinzii as a confounding organism in murine models of pulmonary disease. Comparative Medicine. 2016 Oct 1;66(5):361-366.