Both membrane stretch and fatty acids directly activate large conductance Ca2+-activated K+ channels in vascular smooth muscle cells

Michael T. Kirber, Richard W. Ordway, Lucie H. Clapp, John V. Walsh, Joshua J. Singer

Research output: Contribution to journalArticle

175 Citations (Scopus)

Abstract

Large conductance Ca2+-activated K+ channels in rabbit pulmonary artery smooth muscle cells are activated by membrane stretch and by arachidonic acid and other fatty acids. Activation by stretch appears to occur by a direct effect of stretch on the channel itself or a closely associated component. In excised inside-out patches stretch activation was seen under conditions which precluded possible mechanisms involving cytosolic factors, release of Ca2+ from intracellular stores, or stretch induced transmembrane flux of Ca2+ or other ions potentially capable or activating the channel, Fatty acids also directly activate this channel. Like stretch activation, fatty acid activation occurs in excised inside-out patches in the absence of cytosolic constituents. Moreover, the channel is activated by fatty acids which, unlike arachidonic acid, are not substrates for the cyclo-oxygenase or lypoxygenase pathways, indicating that oxygenated metabolites do not mediate the response. Thus, four distinct types of stimuli (cytosolic Ca2+, membrane potential, membrane stretch, and fatty acids) can directly affect the activity of this channel.

Original languageEnglish (US)
Pages (from-to)24-28
Number of pages5
JournalFEBS Letters
Volume297
Issue number1-2
DOIs
StatePublished - Feb 3 1992

Fingerprint

Calcium-Activated Potassium Channels
Vascular Smooth Muscle
Smooth Muscle Myocytes
Muscle
Fatty Acids
Cells
Membranes
Chemical activation
Arachidonic Acid
Prostaglandin-Endoperoxide Synthases
Metabolites
Membrane Potentials
Pulmonary Artery
Cell Membrane
Ions
Fluxes
Rabbits
Substrates

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Kirber, Michael T. ; Ordway, Richard W. ; Clapp, Lucie H. ; Walsh, John V. ; Singer, Joshua J. / Both membrane stretch and fatty acids directly activate large conductance Ca2+-activated K+ channels in vascular smooth muscle cells. In: FEBS Letters. 1992 ; Vol. 297, No. 1-2. pp. 24-28.
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Both membrane stretch and fatty acids directly activate large conductance Ca2+-activated K+ channels in vascular smooth muscle cells. / Kirber, Michael T.; Ordway, Richard W.; Clapp, Lucie H.; Walsh, John V.; Singer, Joshua J.

In: FEBS Letters, Vol. 297, No. 1-2, 03.02.1992, p. 24-28.

Research output: Contribution to journalArticle

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T1 - Both membrane stretch and fatty acids directly activate large conductance Ca2+-activated K+ channels in vascular smooth muscle cells

AU - Kirber, Michael T.

AU - Ordway, Richard W.

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AU - Singer, Joshua J.

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AB - Large conductance Ca2+-activated K+ channels in rabbit pulmonary artery smooth muscle cells are activated by membrane stretch and by arachidonic acid and other fatty acids. Activation by stretch appears to occur by a direct effect of stretch on the channel itself or a closely associated component. In excised inside-out patches stretch activation was seen under conditions which precluded possible mechanisms involving cytosolic factors, release of Ca2+ from intracellular stores, or stretch induced transmembrane flux of Ca2+ or other ions potentially capable or activating the channel, Fatty acids also directly activate this channel. Like stretch activation, fatty acid activation occurs in excised inside-out patches in the absence of cytosolic constituents. Moreover, the channel is activated by fatty acids which, unlike arachidonic acid, are not substrates for the cyclo-oxygenase or lypoxygenase pathways, indicating that oxygenated metabolites do not mediate the response. Thus, four distinct types of stimuli (cytosolic Ca2+, membrane potential, membrane stretch, and fatty acids) can directly affect the activity of this channel.

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