Both upstream and downstream intergenic regions are critical for the mob as tumor suppressor gene activity in Drosophila

Yongfei Yang, Vikas Gupta, Li Lun Ho, Bo Zhou, Qichang Fan, Zuoyan Zhu, Wenxia Zhang, Zhi-chun Lai

Research output: Contribution to journalArticle

Abstract

The Drosophila mats gene plays a critical role in growth control. Using molecular genetic approaches we investigated how mats is regulated in development. A 2236-bp genomic sequence that contains entire mats including upstream and downstream intergenic regions can rescue mats mutant phenotypes, indicating that regulatory elements necessary for proper mats expression are mostly retained. However, constructs without the upstream or downstream intergenic region failed to rescue mats mutants, demonstrating the functional importance of these sequences. Moreover, mats expression is reduced in matse17, a mats allele with over one-third of the downstream intergenic region deleted. Consistent with a model that the downstream intergenic region is critical for mats activity, this sequence contains evolutionarily conserved elements and has enhancer activities.

Original languageEnglish (US)
Pages (from-to)1766-1770
Number of pages5
JournalFEBS Letters
Volume582
Issue number12
DOIs
StatePublished - May 28 2008

Fingerprint

Intergenic DNA
Tumor Suppressor Genes
Drosophila
Tumors
Genes
Molecular Biology
Alleles
Phenotype
Growth

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Yang, Yongfei ; Gupta, Vikas ; Ho, Li Lun ; Zhou, Bo ; Fan, Qichang ; Zhu, Zuoyan ; Zhang, Wenxia ; Lai, Zhi-chun. / Both upstream and downstream intergenic regions are critical for the mob as tumor suppressor gene activity in Drosophila. In: FEBS Letters. 2008 ; Vol. 582, No. 12. pp. 1766-1770.
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Both upstream and downstream intergenic regions are critical for the mob as tumor suppressor gene activity in Drosophila. / Yang, Yongfei; Gupta, Vikas; Ho, Li Lun; Zhou, Bo; Fan, Qichang; Zhu, Zuoyan; Zhang, Wenxia; Lai, Zhi-chun.

In: FEBS Letters, Vol. 582, No. 12, 28.05.2008, p. 1766-1770.

Research output: Contribution to journalArticle

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AU - Yang, Yongfei

AU - Gupta, Vikas

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AU - Zhu, Zuoyan

AU - Zhang, Wenxia

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