Breakdown of self-incompatibility in a natural population of Petunia axillaris (Solanaceae) in Uruguay containing both self-incompatible and self-compatible plants

Tatsuya Tsukamoto, Toshio Ando, Hisashi Kokubun, Hitoshi Watanabe, Masahiro Masada, Xia Zhu, Eduardo Marchesi, Teh-hui Kao

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Many members of the Solanaceae display a type of gametophytic self-incompatibility which is controlled by a single multiallelic locus, called the S-locus. From our previous survey of more than 100 natural populations of Petunia axillaris (a solanaceous species) in Uruguay, we had found that the majority of the populations of subspecies axillaris were comprised of virtually all self-incompatible individuals. The rest were 'mixed populations' which contained mostly self-incompatible and some self-compatible individuals. In this study, we examined the self-incompatibility behavior and determined the S-genotypes of 33 plants raised from seeds obtained from one such mixed population, designated U1. We found that 30 of the 33 plants (designated U1-1 through U1-33) were self-incompatible and a total of 18 different S-alleles were represented. To determine the S-genotypes of the three self-compatible plants (U1-2, U1-16, and U1-22) and the possible causes for the breakdown of their self-incompatibility, we carried out reciprocal crosses between each of them and each of the 18 S-homozygotes (S1S1 through S18S18) obtained from bud-selfed progeny of 14 of the 30 self-incompatible plants. For U1-2 and U1-16, we also carried out additional crosses with U1-25 (with S1S13 genotype) and an S13S15 plant (obtained from a cross between an S13-homozygote and an S15-homozygote), respectively. Based on all the pollination results and analysis of the production of S-RNases, products of S-alleles in the pistil, we determined the S-genotypes of U1-2, U1-16, and U1-22, and propose that the breakdown of self-incompatibility in these three plants is caused by suppression of the production of S13-RNase from the S13-allele they all carry. We have termed this phenomenon 'stylar-part suppression of an S-allele' or SPS.

Original languageEnglish (US)
Pages (from-to)6-13
Number of pages8
JournalSexual Plant Reproduction
Volume12
Issue number1
DOIs
StatePublished - Jan 1 1999

Fingerprint

Petunia axillaris
Petunia
Solanaceae
Uruguay
5-chloro-3-tert-butyl-2'-chloro-4'-nitrosalicylanilide
Homozygote
homozygosity
Alleles
Genotype
Population
alleles
genotype
Pollination
loci
pistil
reciprocal crosses
Ribonucleases
Seeds
pollination
buds

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Tsukamoto, Tatsuya ; Ando, Toshio ; Kokubun, Hisashi ; Watanabe, Hitoshi ; Masada, Masahiro ; Zhu, Xia ; Marchesi, Eduardo ; Kao, Teh-hui. / Breakdown of self-incompatibility in a natural population of Petunia axillaris (Solanaceae) in Uruguay containing both self-incompatible and self-compatible plants. In: Sexual Plant Reproduction. 1999 ; Vol. 12, No. 1. pp. 6-13.
@article{6a063803cd2d4a6f993891f33384e64f,
title = "Breakdown of self-incompatibility in a natural population of Petunia axillaris (Solanaceae) in Uruguay containing both self-incompatible and self-compatible plants",
abstract = "Many members of the Solanaceae display a type of gametophytic self-incompatibility which is controlled by a single multiallelic locus, called the S-locus. From our previous survey of more than 100 natural populations of Petunia axillaris (a solanaceous species) in Uruguay, we had found that the majority of the populations of subspecies axillaris were comprised of virtually all self-incompatible individuals. The rest were 'mixed populations' which contained mostly self-incompatible and some self-compatible individuals. In this study, we examined the self-incompatibility behavior and determined the S-genotypes of 33 plants raised from seeds obtained from one such mixed population, designated U1. We found that 30 of the 33 plants (designated U1-1 through U1-33) were self-incompatible and a total of 18 different S-alleles were represented. To determine the S-genotypes of the three self-compatible plants (U1-2, U1-16, and U1-22) and the possible causes for the breakdown of their self-incompatibility, we carried out reciprocal crosses between each of them and each of the 18 S-homozygotes (S1S1 through S18S18) obtained from bud-selfed progeny of 14 of the 30 self-incompatible plants. For U1-2 and U1-16, we also carried out additional crosses with U1-25 (with S1S13 genotype) and an S13S15 plant (obtained from a cross between an S13-homozygote and an S15-homozygote), respectively. Based on all the pollination results and analysis of the production of S-RNases, products of S-alleles in the pistil, we determined the S-genotypes of U1-2, U1-16, and U1-22, and propose that the breakdown of self-incompatibility in these three plants is caused by suppression of the production of S13-RNase from the S13-allele they all carry. We have termed this phenomenon 'stylar-part suppression of an S-allele' or SPS.",
author = "Tatsuya Tsukamoto and Toshio Ando and Hisashi Kokubun and Hitoshi Watanabe and Masahiro Masada and Xia Zhu and Eduardo Marchesi and Teh-hui Kao",
year = "1999",
month = "1",
day = "1",
doi = "10.1007/s004970050166",
language = "English (US)",
volume = "12",
pages = "6--13",
journal = "Plant Reproduction",
issn = "2194-7953",
publisher = "Springer Verlag",
number = "1",

}

Breakdown of self-incompatibility in a natural population of Petunia axillaris (Solanaceae) in Uruguay containing both self-incompatible and self-compatible plants. / Tsukamoto, Tatsuya; Ando, Toshio; Kokubun, Hisashi; Watanabe, Hitoshi; Masada, Masahiro; Zhu, Xia; Marchesi, Eduardo; Kao, Teh-hui.

In: Sexual Plant Reproduction, Vol. 12, No. 1, 01.01.1999, p. 6-13.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Breakdown of self-incompatibility in a natural population of Petunia axillaris (Solanaceae) in Uruguay containing both self-incompatible and self-compatible plants

AU - Tsukamoto, Tatsuya

AU - Ando, Toshio

AU - Kokubun, Hisashi

AU - Watanabe, Hitoshi

AU - Masada, Masahiro

AU - Zhu, Xia

AU - Marchesi, Eduardo

AU - Kao, Teh-hui

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Many members of the Solanaceae display a type of gametophytic self-incompatibility which is controlled by a single multiallelic locus, called the S-locus. From our previous survey of more than 100 natural populations of Petunia axillaris (a solanaceous species) in Uruguay, we had found that the majority of the populations of subspecies axillaris were comprised of virtually all self-incompatible individuals. The rest were 'mixed populations' which contained mostly self-incompatible and some self-compatible individuals. In this study, we examined the self-incompatibility behavior and determined the S-genotypes of 33 plants raised from seeds obtained from one such mixed population, designated U1. We found that 30 of the 33 plants (designated U1-1 through U1-33) were self-incompatible and a total of 18 different S-alleles were represented. To determine the S-genotypes of the three self-compatible plants (U1-2, U1-16, and U1-22) and the possible causes for the breakdown of their self-incompatibility, we carried out reciprocal crosses between each of them and each of the 18 S-homozygotes (S1S1 through S18S18) obtained from bud-selfed progeny of 14 of the 30 self-incompatible plants. For U1-2 and U1-16, we also carried out additional crosses with U1-25 (with S1S13 genotype) and an S13S15 plant (obtained from a cross between an S13-homozygote and an S15-homozygote), respectively. Based on all the pollination results and analysis of the production of S-RNases, products of S-alleles in the pistil, we determined the S-genotypes of U1-2, U1-16, and U1-22, and propose that the breakdown of self-incompatibility in these three plants is caused by suppression of the production of S13-RNase from the S13-allele they all carry. We have termed this phenomenon 'stylar-part suppression of an S-allele' or SPS.

AB - Many members of the Solanaceae display a type of gametophytic self-incompatibility which is controlled by a single multiallelic locus, called the S-locus. From our previous survey of more than 100 natural populations of Petunia axillaris (a solanaceous species) in Uruguay, we had found that the majority of the populations of subspecies axillaris were comprised of virtually all self-incompatible individuals. The rest were 'mixed populations' which contained mostly self-incompatible and some self-compatible individuals. In this study, we examined the self-incompatibility behavior and determined the S-genotypes of 33 plants raised from seeds obtained from one such mixed population, designated U1. We found that 30 of the 33 plants (designated U1-1 through U1-33) were self-incompatible and a total of 18 different S-alleles were represented. To determine the S-genotypes of the three self-compatible plants (U1-2, U1-16, and U1-22) and the possible causes for the breakdown of their self-incompatibility, we carried out reciprocal crosses between each of them and each of the 18 S-homozygotes (S1S1 through S18S18) obtained from bud-selfed progeny of 14 of the 30 self-incompatible plants. For U1-2 and U1-16, we also carried out additional crosses with U1-25 (with S1S13 genotype) and an S13S15 plant (obtained from a cross between an S13-homozygote and an S15-homozygote), respectively. Based on all the pollination results and analysis of the production of S-RNases, products of S-alleles in the pistil, we determined the S-genotypes of U1-2, U1-16, and U1-22, and propose that the breakdown of self-incompatibility in these three plants is caused by suppression of the production of S13-RNase from the S13-allele they all carry. We have termed this phenomenon 'stylar-part suppression of an S-allele' or SPS.

UR - http://www.scopus.com/inward/record.url?scp=0032783319&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032783319&partnerID=8YFLogxK

U2 - 10.1007/s004970050166

DO - 10.1007/s004970050166

M3 - Article

VL - 12

SP - 6

EP - 13

JO - Plant Reproduction

JF - Plant Reproduction

SN - 2194-7953

IS - 1

ER -