Breakdown of self-incompatibility in a natural population of Petunia axillaris caused by a modifier locus that suppresses the expression of an S-RNase gene

Tatsuya Tsukamoto, Toshio Ando, Hisashi Kokubun, Hitoshi Watanabe, Takahide Sato, Masahiro Masada, Eduardo Marchesi, Teh Hui Kao

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We had previously studied the cause of breakdown of self-incompatibility in a natural population (designated U1) of Petunia axillaris subsp. axillaris (Solanaceae) identified in Uruguay. This population contained mostly self-incompatible and some self-compatible plants. We had found that an S-haplotype, S13, carried by three self-compatible individuals (U1-2, U1-16, and U1-22) was functional in the pollen, but not in the style, and that S13-RNase was not detected in the styles of these individuals. This defective haplotype was designated S13sps, with "sps" standing for "stylar-part suppression". In this work, we further investigated the molecular and genetic basis of this suppression. We isolated and sequenced cDNAs for S13-, S1- and S15-RNases, and used them as probes to show that the transcript of the S13-RNase gene was not detectable in U1-2 (S1S13sps), U1-16 (S13spsS15), or U1-22 (S13spsS13sps), but the transcript levels of the S1- and S15-RNase genes in U1-2 and U1-16, respectively, were normal. Genomic blotting analysis revealed that the S13-RNase gene was present in these three self-compatible plants. The S-genotypes obtained in budselfed and normally selfed progenies of these three plants were determined. Analysis of these S-genotypes led us to propose a model invoking a modifier locus, named MDF, to explain the suppression of the expression of the S13-RNase gene in the genetic background of the self-compatible plants carrying the S13sps-haplotype.

Original languageEnglish (US)
Pages (from-to)255-263
Number of pages9
JournalSexual Plant Reproduction
Volume15
Issue number5
StatePublished - Jan 1 2003

Fingerprint

Petunia axillaris
5-chloro-3-tert-butyl-2'-chloro-4'-nitrosalicylanilide
Petunia
modifiers (genes)
Ribonucleases
haplotypes
loci
Haplotypes
Population
Genes
genes
genotype
Uruguay
Solanaceae
Genotype
genetic background
Genetic Suppression
pollen
Pollen
genomics

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Tsukamoto, Tatsuya ; Ando, Toshio ; Kokubun, Hisashi ; Watanabe, Hitoshi ; Sato, Takahide ; Masada, Masahiro ; Marchesi, Eduardo ; Kao, Teh Hui. / Breakdown of self-incompatibility in a natural population of Petunia axillaris caused by a modifier locus that suppresses the expression of an S-RNase gene. In: Sexual Plant Reproduction. 2003 ; Vol. 15, No. 5. pp. 255-263.
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abstract = "We had previously studied the cause of breakdown of self-incompatibility in a natural population (designated U1) of Petunia axillaris subsp. axillaris (Solanaceae) identified in Uruguay. This population contained mostly self-incompatible and some self-compatible plants. We had found that an S-haplotype, S13, carried by three self-compatible individuals (U1-2, U1-16, and U1-22) was functional in the pollen, but not in the style, and that S13-RNase was not detected in the styles of these individuals. This defective haplotype was designated S13sps, with {"}sps{"} standing for {"}stylar-part suppression{"}. In this work, we further investigated the molecular and genetic basis of this suppression. We isolated and sequenced cDNAs for S13-, S1- and S15-RNases, and used them as probes to show that the transcript of the S13-RNase gene was not detectable in U1-2 (S1S13sps), U1-16 (S13spsS15), or U1-22 (S13spsS13sps), but the transcript levels of the S1- and S15-RNase genes in U1-2 and U1-16, respectively, were normal. Genomic blotting analysis revealed that the S13-RNase gene was present in these three self-compatible plants. The S-genotypes obtained in budselfed and normally selfed progenies of these three plants were determined. Analysis of these S-genotypes led us to propose a model invoking a modifier locus, named MDF, to explain the suppression of the expression of the S13-RNase gene in the genetic background of the self-compatible plants carrying the S13sps-haplotype.",
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Breakdown of self-incompatibility in a natural population of Petunia axillaris caused by a modifier locus that suppresses the expression of an S-RNase gene. / Tsukamoto, Tatsuya; Ando, Toshio; Kokubun, Hisashi; Watanabe, Hitoshi; Sato, Takahide; Masada, Masahiro; Marchesi, Eduardo; Kao, Teh Hui.

In: Sexual Plant Reproduction, Vol. 15, No. 5, 01.01.2003, p. 255-263.

Research output: Contribution to journalArticle

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AU - Tsukamoto, Tatsuya

AU - Ando, Toshio

AU - Kokubun, Hisashi

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AU - Kao, Teh Hui

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