Breast and abdominal adipose multipotent mesenchymal stromal cells and stage-specific embryonic antigen 4 expression

Jacquelyn R. Maddox, Katherine D. Ludlow, Feng Li, Christopher Niyibizi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We assessed human mesenchymal stem cells (MSCs) harvested from breast and abdominal adipose tissues enriched in embryonic stage-specific antigen (SSEA-4) expression for osteogenic and adipogenic differentiation in comparison to a mixed cell population. Human adipose was obtained from abdominal and breast tissues of females undergoing gastric bypass and breast reduction, respectively. SSEA-4-expressing cells were enriched from the mixed cell population by magnetic cell sorting and expanded in culture. The results showed that freshly isolated cells from breast and abdominal tissues based on adipose from 3 patients comprised 12 and 10% SSEA-4+ cells, respectively. At passage 0, 48% of the cells from breast adipose tissue were positive for SSEA-4 while 12% of the cells from abdominal adipose tissue were positive for this antigen. The level of SSEA-4-expressing cells remained relatively constant with passaging; SSEA-4-expressing cells from breast tissue comprised 45% of the total while 27% of the cells from abdominal adipose tissue expressed SSEA-4 at passage 5. Cells sorted for SSEA-4 expression exhibited a higher potential for differentiation toward osteogenic and adipogenc cell lineages in vitro when compared to a mixed population. Interestingly, SSEA-4 expression was lost upon differentiation, suggesting that the antigen marks a subpopulation of MSCs. Taken together, the data demonstrate that breast adipose tissue is highly enriched in a subpopulation of MSCs expressing SSEA-4 and suggest that SSEA-4 may be a marker of a subpopulation of MSCs with high potential for osteogenic and adipogenic differentiation.

Original languageEnglish (US)
Pages (from-to)107-116
Number of pages10
JournalCells Tissues Organs
Volume196
Issue number2
DOIs
StatePublished - Jun 1 2012

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Mesenchymal Stromal Cells
Breast
Abdominal Fat
stage-specific embryonic antigen-4
Adipose Tissue
Stage-Specific Embryonic Antigens
Population
Gastric Bypass
Differentiation Antigens
Cell Lineage
Antigens

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology

Cite this

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title = "Breast and abdominal adipose multipotent mesenchymal stromal cells and stage-specific embryonic antigen 4 expression",
abstract = "We assessed human mesenchymal stem cells (MSCs) harvested from breast and abdominal adipose tissues enriched in embryonic stage-specific antigen (SSEA-4) expression for osteogenic and adipogenic differentiation in comparison to a mixed cell population. Human adipose was obtained from abdominal and breast tissues of females undergoing gastric bypass and breast reduction, respectively. SSEA-4-expressing cells were enriched from the mixed cell population by magnetic cell sorting and expanded in culture. The results showed that freshly isolated cells from breast and abdominal tissues based on adipose from 3 patients comprised 12 and 10{\%} SSEA-4+ cells, respectively. At passage 0, 48{\%} of the cells from breast adipose tissue were positive for SSEA-4 while 12{\%} of the cells from abdominal adipose tissue were positive for this antigen. The level of SSEA-4-expressing cells remained relatively constant with passaging; SSEA-4-expressing cells from breast tissue comprised 45{\%} of the total while 27{\%} of the cells from abdominal adipose tissue expressed SSEA-4 at passage 5. Cells sorted for SSEA-4 expression exhibited a higher potential for differentiation toward osteogenic and adipogenc cell lineages in vitro when compared to a mixed population. Interestingly, SSEA-4 expression was lost upon differentiation, suggesting that the antigen marks a subpopulation of MSCs. Taken together, the data demonstrate that breast adipose tissue is highly enriched in a subpopulation of MSCs expressing SSEA-4 and suggest that SSEA-4 may be a marker of a subpopulation of MSCs with high potential for osteogenic and adipogenic differentiation.",
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Breast and abdominal adipose multipotent mesenchymal stromal cells and stage-specific embryonic antigen 4 expression. / Maddox, Jacquelyn R.; Ludlow, Katherine D.; Li, Feng; Niyibizi, Christopher.

In: Cells Tissues Organs, Vol. 196, No. 2, 01.06.2012, p. 107-116.

Research output: Contribution to journalArticle

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