Brefeldin A inhibits protein synthesis through the phosphorylation of the α-subunit of eukaryotic initiation factor-2

Harry Mellor; Scot R. Kimball; Leonard S. Jefferson

doi:10.1016/0014-5793(94)00756-X

Brefeldin A inhibits protein synthesis through the phosphorylation of the α-subunit of eukaryotic initiation factor-2

Harry Mellor, Scot R. Kimball, Leonard S. Jefferson

Department of Cellular and Molecular Physiology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Brefeldin A is a fungal metabolite which disrupts protein traffic through the Golgi apparatus and thereby inhibits protein secretion. Recently, it has been shown that Brefeldin A also causes a marked decrease in the rate of protein synthesis in cells in culture [1992, FEBS Lett. 314, 371-374]. We show here that treatment of rat GH3 pituitary cells with Brefeldin A leads to an inhibition of protein synthesis at the level of peptide-chain initiation through a mechanism involving the phosphorylation of the α-subunit of eukaryotic initiation factor-2 (eIF-2α).

Original language	English (US)
Pages (from-to)	143-146
Number of pages	4
Journal	FEBS Letters
Volume	350
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(94)00756-X
State	Published - Aug 15 1994

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(94)00756-X

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title = "Brefeldin A inhibits protein synthesis through the phosphorylation of the α-subunit of eukaryotic initiation factor-2",

abstract = "Brefeldin A is a fungal metabolite which disrupts protein traffic through the Golgi apparatus and thereby inhibits protein secretion. Recently, it has been shown that Brefeldin A also causes a marked decrease in the rate of protein synthesis in cells in culture [1992, FEBS Lett. 314, 371-374]. We show here that treatment of rat GH3 pituitary cells with Brefeldin A leads to an inhibition of protein synthesis at the level of peptide-chain initiation through a mechanism involving the phosphorylation of the α-subunit of eukaryotic initiation factor-2 (eIF-2α).",

author = "Harry Mellor and Kimball, {Scot R.} and Jefferson, {Leonard S.}",

note = "Funding Information: AcknowledgementsT: he authors would like to thank Joan B. McGwire and Lynne C. Pletcher for their excellentt echnical assistance.T his work was supported by Grants DK13499 and DKl.5658 from the National Institutes of Health. Harry Mellor was supported by Training Grant DK07684 from the National Institutes of Health.",

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doi = "10.1016/0014-5793(94)00756-X",

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T1 - Brefeldin A inhibits protein synthesis through the phosphorylation of the α-subunit of eukaryotic initiation factor-2

AU - Mellor, Harry

AU - Kimball, Scot R.

AU - Jefferson, Leonard S.

N1 - Funding Information: AcknowledgementsT: he authors would like to thank Joan B. McGwire and Lynne C. Pletcher for their excellentt echnical assistance.T his work was supported by Grants DK13499 and DKl.5658 from the National Institutes of Health. Harry Mellor was supported by Training Grant DK07684 from the National Institutes of Health.

PY - 1994/8/15

Y1 - 1994/8/15

N2 - Brefeldin A is a fungal metabolite which disrupts protein traffic through the Golgi apparatus and thereby inhibits protein secretion. Recently, it has been shown that Brefeldin A also causes a marked decrease in the rate of protein synthesis in cells in culture [1992, FEBS Lett. 314, 371-374]. We show here that treatment of rat GH3 pituitary cells with Brefeldin A leads to an inhibition of protein synthesis at the level of peptide-chain initiation through a mechanism involving the phosphorylation of the α-subunit of eukaryotic initiation factor-2 (eIF-2α).

AB - Brefeldin A is a fungal metabolite which disrupts protein traffic through the Golgi apparatus and thereby inhibits protein secretion. Recently, it has been shown that Brefeldin A also causes a marked decrease in the rate of protein synthesis in cells in culture [1992, FEBS Lett. 314, 371-374]. We show here that treatment of rat GH3 pituitary cells with Brefeldin A leads to an inhibition of protein synthesis at the level of peptide-chain initiation through a mechanism involving the phosphorylation of the α-subunit of eukaryotic initiation factor-2 (eIF-2α).

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Brefeldin A inhibits protein synthesis through the phosphorylation of the α-subunit of eukaryotic initiation factor-2

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