Brief anesthesia, but not voluntary locomotion, significantly alters cortical temperature

Michael J. Shirey, Jared B. Smith, D’Anne E. Kudlik, Bing Xing Huo, Stephanie E. Greene, Patrick J. Drew

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Changes in brain temperature can alter electrical properties of neurons and cause changes in behavior. However, it is not well understood how behaviors, like locomotion, or experimental manipulations, like anesthesia, alter brain temperature. We implanted thermocouples in sensorimotor cortex of mice to understand how cortical temperature was affected by locomotion, as well as by brief and prolonged anesthesia. Voluntary locomotion induced small (~0.1°C) but reliable increases in cortical temperature that could be described using a linear convolution model. In contrast, brief (90-s) exposure to isoflurane anesthesia depressed cortical temperature by ~2°C, which lasted for up to 30 min after the cessation of anesthesia. Cortical temperature decreases were not accompanied by a concomitant decrease in the γ-band local field potential power, multiunit firing rate, or locomotion behavior, which all returned to baseline within a few minutes after the cessation of anesthesia. In anesthetized animals where core body temperature was kept constant, cortical temperature was still >1°C lower than in the awake animal. Thermocouples implanted in the subcortex showed similar temperature changes under anesthesia, suggesting these responses occur throughout the brain. Two-photon microscopy of individual blood vessel dynamics following brief isoflurane exposure revealed a large increase in vessel diameter that ceased before the brain temperature significantly decreased, indicating cerebral heat loss was not due to increased cerebral blood vessel dilation. These data should be considered in experimental designs recording in anesthetized preparations, computational models relating temperature and neural activity, and awake-behaving methods that require brief anesthesia before experimental procedures.

Original languageEnglish (US)
Pages (from-to)309-322
Number of pages14
JournalJournal of neurophysiology
Volume114
Issue number1
DOIs
StatePublished - May 13 2015

Fingerprint

Locomotion
Anesthesia
Temperature
Isoflurane
Brain
Blood Vessels
Body Temperature
Photons
Dilatation
Microscopy
Linear Models
Research Design
Hot Temperature
Neurons

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Physiology

Cite this

Shirey, Michael J. ; Smith, Jared B. ; Kudlik, D’Anne E. ; Huo, Bing Xing ; Greene, Stephanie E. ; Drew, Patrick J. / Brief anesthesia, but not voluntary locomotion, significantly alters cortical temperature. In: Journal of neurophysiology. 2015 ; Vol. 114, No. 1. pp. 309-322.
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Brief anesthesia, but not voluntary locomotion, significantly alters cortical temperature. / Shirey, Michael J.; Smith, Jared B.; Kudlik, D’Anne E.; Huo, Bing Xing; Greene, Stephanie E.; Drew, Patrick J.

In: Journal of neurophysiology, Vol. 114, No. 1, 13.05.2015, p. 309-322.

Research output: Contribution to journalArticle

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