BSF1, a novel brain-specific DNA-binding protein recognizing a tandemly repeated purine DNA element in the GABA(A) receptor δ subunit gene

K. Motejlek, R. Hauselmann, S. Leitgeb, Bernhard Luscher

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28 Scopus citations

Abstract

The rat GABA(A) receptor δ subunit gene has been isolated and its promoter mapped to facilitate studies of neuron-specific gene transcription. The gene is transcribed from a single promoter with several clustered transcriptional initiation sites. The major start site maps to a prototype initiator sequence located in the center of a TATA-less CpG-rich island. Analysis of the 5'-flanking region of the GABA(A) receptor δ subunit gene revealed a novel tandemly repeated purine sequence element. Furthermore, gel retardation assays identified several tissue-specific and ubiquitous proteins that bind to this sequence. One of these factors, brain-specific factor (BSF) 1, was only observed in extracts from brain and was distinct from BETA and PAL, two DNA-binding proteins with similar purine-rich recognition sequences. The distribution of BSF1 in brain closely matched the expression pattern previously determined for the δ subunit mRNA, and its binding activity appeared simultaneously with GABA(A) receptor δ subunit mRNA during differentiation of cerebellar granule cells in vitro. Our data therefore suggest that BSF1 is a novel brain-specific and neuronal cell type-enriched factor possibly involved in differential transcription of the GABA(A) receptor δ subunit gene.

Original languageEnglish (US)
Pages (from-to)15265-15273
Number of pages9
JournalJournal of Biological Chemistry
Volume269
Issue number21
StatePublished - Jan 1 1994

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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