Buffer effects on high affinity [3H]-prazosin binding in brain and spinal cord

D. W. Herr; R. B. Mailman

doi:10.1016/0091-3057(89)90043-9

Buffer effects on high affinity [³H]-prazosin binding in brain and spinal cord

D. W. Herr, R. B. Mailman

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

[³H]-Prazosin binding was characterized in cortical and spinal membranes from Fischer 344N and Sprague-Dawley rats. Estimates of B_max and K_d values were comparable with earlier studies of these regions in the central nervous system (CNS). However, the K_d obtained using Tris buffer system was greater than when HEPES or phosphate buffer was used. These data indicate that high affinity [³H]-prazosin binding in the homogenates of tissue from the CNS is affected critically by buffer selection.

Original language	English (US)
Pages (from-to)	831-834
Number of pages	4
Journal	Pharmacology, Biochemistry and Behavior
Volume	32
Issue number	3
DOIs	https://doi.org/10.1016/0091-3057(89)90043-9
State	Published - Mar 1989

All Science Journal Classification (ASJC) codes

Biochemistry
Toxicology
Pharmacology
Clinical Biochemistry
Biological Psychiatry
Behavioral Neuroscience

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10.1016/0091-3057(89)90043-9

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title = "Buffer effects on high affinity [3H]-prazosin binding in brain and spinal cord",

abstract = "[3H]-Prazosin binding was characterized in cortical and spinal membranes from Fischer 344N and Sprague-Dawley rats. Estimates of Bmax and Kd values were comparable with earlier studies of these regions in the central nervous system (CNS). However, the Kd obtained using Tris buffer system was greater than when HEPES or phosphate buffer was used. These data indicate that high affinity [3H]-prazosin binding in the homogenates of tissue from the CNS is affected critically by buffer selection.",

author = "Herr, {D. W.} and Mailman, {R. B.}",

note = "Funding Information: This work was supportedin part by a predoctoratlr aineeshipto D.W.H. (NationaRl esearchS erviceA ward5 T32 ES-07126f)r om the NationaIln stitutoe f EnvironmentHael althS ciencesa,n db y programgr ant ES-01104 and a BurroughsW ellcomeToxicology Scholar Award (R,B.M.).",

year = "1989",

month = mar,

doi = "10.1016/0091-3057(89)90043-9",

language = "English (US)",

volume = "32",

pages = "831--834",

journal = "Pharmacology Biochemistry and Behavior",

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T1 - Buffer effects on high affinity [3H]-prazosin binding in brain and spinal cord

AU - Herr, D. W.

AU - Mailman, R. B.

N1 - Funding Information: This work was supportedin part by a predoctoratlr aineeshipto D.W.H. (NationaRl esearchS erviceA ward5 T32 ES-07126f)r om the NationaIln stitutoe f EnvironmentHael althS ciencesa,n db y programgr ant ES-01104 and a BurroughsW ellcomeToxicology Scholar Award (R,B.M.).

PY - 1989/3

Y1 - 1989/3

N2 - [3H]-Prazosin binding was characterized in cortical and spinal membranes from Fischer 344N and Sprague-Dawley rats. Estimates of Bmax and Kd values were comparable with earlier studies of these regions in the central nervous system (CNS). However, the Kd obtained using Tris buffer system was greater than when HEPES or phosphate buffer was used. These data indicate that high affinity [3H]-prazosin binding in the homogenates of tissue from the CNS is affected critically by buffer selection.

AB - [3H]-Prazosin binding was characterized in cortical and spinal membranes from Fischer 344N and Sprague-Dawley rats. Estimates of Bmax and Kd values were comparable with earlier studies of these regions in the central nervous system (CNS). However, the Kd obtained using Tris buffer system was greater than when HEPES or phosphate buffer was used. These data indicate that high affinity [3H]-prazosin binding in the homogenates of tissue from the CNS is affected critically by buffer selection.

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JO - Pharmacology Biochemistry and Behavior

JF - Pharmacology Biochemistry and Behavior

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ER -

Buffer effects on high affinity [³H]-prazosin binding in brain and spinal cord

Abstract

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