Butryic acid analogues augment γ globin gene expression in neonatal erythroid progenitors

S. P. Perrine, Barbara Miller, M. F. Greene, R. A. Cohen, N. Cook, C. Shackleton, D. V. Faller

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The γ→β globin gene switch in humans is normally on a set developmental clock but is delayed in infants of diabetic mothers. We cultured cord blood erythroid progenitors and assayed globin produced in the presence and absence of metabolites that are elevated in such infants. Analogues of butyric acid at supranormal concentrations significantly augmented γ and inhibited β globin expression. The uptake of α-amino-n-butyric acid into colony-derived erythroblasts was increased in the presence of supranormal insulin. These findings suggest that elevated levels of α-amino-n-butyric acid and insulin in the developing fetus delay the globin switch and may offer potential for augmenting γ globin expression in the β globin chain diseases.

Original languageEnglish (US)
Pages (from-to)694-700
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume148
Issue number2
DOIs
StatePublished - Oct 29 1987

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Globins
Gene expression
Gene Expression
Acids
Butyric Acid
Switches
Switch Genes
Insulin
Erythroblasts
Metabolites
Fetal Blood
Clocks
Fetus
Blood
Genes
Mothers

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Perrine, S. P. ; Miller, Barbara ; Greene, M. F. ; Cohen, R. A. ; Cook, N. ; Shackleton, C. ; Faller, D. V. / Butryic acid analogues augment γ globin gene expression in neonatal erythroid progenitors. In: Biochemical and Biophysical Research Communications. 1987 ; Vol. 148, No. 2. pp. 694-700.
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Butryic acid analogues augment γ globin gene expression in neonatal erythroid progenitors. / Perrine, S. P.; Miller, Barbara; Greene, M. F.; Cohen, R. A.; Cook, N.; Shackleton, C.; Faller, D. V.

In: Biochemical and Biophysical Research Communications, Vol. 148, No. 2, 29.10.1987, p. 694-700.

Research output: Contribution to journalArticle

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AU - Perrine, S. P.

AU - Miller, Barbara

AU - Greene, M. F.

AU - Cohen, R. A.

AU - Cook, N.

AU - Shackleton, C.

AU - Faller, D. V.

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AB - The γ→β globin gene switch in humans is normally on a set developmental clock but is delayed in infants of diabetic mothers. We cultured cord blood erythroid progenitors and assayed globin produced in the presence and absence of metabolites that are elevated in such infants. Analogues of butyric acid at supranormal concentrations significantly augmented γ and inhibited β globin expression. The uptake of α-amino-n-butyric acid into colony-derived erythroblasts was increased in the presence of supranormal insulin. These findings suggest that elevated levels of α-amino-n-butyric acid and insulin in the developing fetus delay the globin switch and may offer potential for augmenting γ globin expression in the β globin chain diseases.

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