C4 genes of the chimpanzee, gorilla, and orang-utan: evidence for extensive homogenization

Hiroshi Kawaguchi, Zofia Zaleska-Rutczynska, Felipe Figueroa, Colm O'hUigin, Jan Klein

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The human complement component 4 is encoded in two genes, C4A and C4B, residing between the class I and class II genes of the major histocompatibility complex. The C4A and C4B molecules differ in their biological activity, the former binding more efficiently to proteins than to carbohydrates while for the latter, the opposite holds true. To shed light on the origin of the C4 genes we isolated cosmid clones bearing the C4 genes of a chimpanzee, a gorilla, and an orang-utan. From the clones, we isolated the fragments coding for the C4d part of the gene (exons and introns) and sequenced them. Altogether we sequenced eight gene fragments: three chimpanzee (Patr-C4-1*01, Patr-C4-1*02, Patr-C4-2*01), two gorilla (Gogo-C4-1*01, Gogo-C4-2*01), and three orang-utan (Popy-C4-1*01, Popy-C4-2*01, Popy-C4-3*01). Comparison of the sequences with each other and with human C4 sequences revealed that in the region believed to be responsible for the functional difference between the C4A and C4B proteins the C4A genes of the different species fell into one group and the C4B genes fell into another. In the rest of the sequence, however, the C4A and C4B genes of each species resembled each other more than they did C4 genes of other species. These results are interpreted as suggesting extensive homogenization (concerted evolution) of the C4 genes in each species, most likely by repeated unequal, homologous, intragenic crossing-over.

Original languageEnglish (US)
Pages (from-to)16-23
Number of pages8
JournalImmunogenetics
Volume35
Issue number1
DOIs
StatePublished - Jan 1992

Fingerprint

Gorilla gorilla
Pan troglodytes
Genes
Clone Cells
Complement C4
Cosmids
MHC Class II Genes
Major Histocompatibility Complex
Introns
Exons
Proteins
Carbohydrates

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Kawaguchi, H., Zaleska-Rutczynska, Z., Figueroa, F., O'hUigin, C., & Klein, J. (1992). C4 genes of the chimpanzee, gorilla, and orang-utan: evidence for extensive homogenization. Immunogenetics, 35(1), 16-23. https://doi.org/10.1007/BF00216622
Kawaguchi, Hiroshi ; Zaleska-Rutczynska, Zofia ; Figueroa, Felipe ; O'hUigin, Colm ; Klein, Jan. / C4 genes of the chimpanzee, gorilla, and orang-utan : evidence for extensive homogenization. In: Immunogenetics. 1992 ; Vol. 35, No. 1. pp. 16-23.
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abstract = "The human complement component 4 is encoded in two genes, C4A and C4B, residing between the class I and class II genes of the major histocompatibility complex. The C4A and C4B molecules differ in their biological activity, the former binding more efficiently to proteins than to carbohydrates while for the latter, the opposite holds true. To shed light on the origin of the C4 genes we isolated cosmid clones bearing the C4 genes of a chimpanzee, a gorilla, and an orang-utan. From the clones, we isolated the fragments coding for the C4d part of the gene (exons and introns) and sequenced them. Altogether we sequenced eight gene fragments: three chimpanzee (Patr-C4-1*01, Patr-C4-1*02, Patr-C4-2*01), two gorilla (Gogo-C4-1*01, Gogo-C4-2*01), and three orang-utan (Popy-C4-1*01, Popy-C4-2*01, Popy-C4-3*01). Comparison of the sequences with each other and with human C4 sequences revealed that in the region believed to be responsible for the functional difference between the C4A and C4B proteins the C4A genes of the different species fell into one group and the C4B genes fell into another. In the rest of the sequence, however, the C4A and C4B genes of each species resembled each other more than they did C4 genes of other species. These results are interpreted as suggesting extensive homogenization (concerted evolution) of the C4 genes in each species, most likely by repeated unequal, homologous, intragenic crossing-over.",
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Kawaguchi, H, Zaleska-Rutczynska, Z, Figueroa, F, O'hUigin, C & Klein, J 1992, 'C4 genes of the chimpanzee, gorilla, and orang-utan: evidence for extensive homogenization', Immunogenetics, vol. 35, no. 1, pp. 16-23. https://doi.org/10.1007/BF00216622

C4 genes of the chimpanzee, gorilla, and orang-utan : evidence for extensive homogenization. / Kawaguchi, Hiroshi; Zaleska-Rutczynska, Zofia; Figueroa, Felipe; O'hUigin, Colm; Klein, Jan.

In: Immunogenetics, Vol. 35, No. 1, 01.1992, p. 16-23.

Research output: Contribution to journalArticle

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T1 - C4 genes of the chimpanzee, gorilla, and orang-utan

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AU - Kawaguchi, Hiroshi

AU - Zaleska-Rutczynska, Zofia

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AU - O'hUigin, Colm

AU - Klein, Jan

PY - 1992/1

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N2 - The human complement component 4 is encoded in two genes, C4A and C4B, residing between the class I and class II genes of the major histocompatibility complex. The C4A and C4B molecules differ in their biological activity, the former binding more efficiently to proteins than to carbohydrates while for the latter, the opposite holds true. To shed light on the origin of the C4 genes we isolated cosmid clones bearing the C4 genes of a chimpanzee, a gorilla, and an orang-utan. From the clones, we isolated the fragments coding for the C4d part of the gene (exons and introns) and sequenced them. Altogether we sequenced eight gene fragments: three chimpanzee (Patr-C4-1*01, Patr-C4-1*02, Patr-C4-2*01), two gorilla (Gogo-C4-1*01, Gogo-C4-2*01), and three orang-utan (Popy-C4-1*01, Popy-C4-2*01, Popy-C4-3*01). Comparison of the sequences with each other and with human C4 sequences revealed that in the region believed to be responsible for the functional difference between the C4A and C4B proteins the C4A genes of the different species fell into one group and the C4B genes fell into another. In the rest of the sequence, however, the C4A and C4B genes of each species resembled each other more than they did C4 genes of other species. These results are interpreted as suggesting extensive homogenization (concerted evolution) of the C4 genes in each species, most likely by repeated unequal, homologous, intragenic crossing-over.

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Kawaguchi H, Zaleska-Rutczynska Z, Figueroa F, O'hUigin C, Klein J. C4 genes of the chimpanzee, gorilla, and orang-utan: evidence for extensive homogenization. Immunogenetics. 1992 Jan;35(1):16-23. https://doi.org/10.1007/BF00216622