Abstract
Astrocytes release ATP and glutamate through vesicular exocytosis to mediate neuron-glial interactions. In contrast to exocytosis, the endocytic pathways in astroglial cells are poorly understood. Here, we identify a constitutive endocytic pathway in cultured astrocytes that is dependent on neither clathrin nor dynamin. This dynamin-independent endocytic pathway is regulated by Rab5, an early endosome protein. The endocytosed vesicles show fast transition from early endosomes to late endosomes and lysosomes within a few minutes. Interestingly, this clathrin-and dynamin-independent endocytosis in astrocytes is potently regulated by intracellular Ca2+. ATP and glutamate greatly enhance the dynamin-independent endocytosis through elevating the intracellular Ca2+. In addition, amyloid-β peptide (Aβ) also enhances the dynamin-independent endocytosis by inducing Ca2+ transients in astrocytes. These results demonstrate a novel endocytic pathway in glial cells that is dynamin independent but tightly regulated by intracellular Ca2+. The regulation by ATP, glutamate, and Aβ suggests an important role of the dynamin-independent endocytosis in both physiological and pathological conditions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 8063-8074 |
| Number of pages | 12 |
| Journal | Journal of Neuroscience |
| Volume | 29 |
| Issue number | 25 |
| DOIs | |
| State | Published - Jun 24 2009 |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
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Ca2+ regulation of dynamin-independent endocytosis in cortical astrocytes. / Jiang, Min; Chen, Gong.
In: Journal of Neuroscience, Vol. 29, No. 25, 24.06.2009, p. 8063-8074.Research output: Contribution to journal › Article
TY - JOUR
T1 - Ca2+ regulation of dynamin-independent endocytosis in cortical astrocytes
AU - Jiang, Min
AU - Chen, Gong
PY - 2009/6/24
Y1 - 2009/6/24
N2 - Astrocytes release ATP and glutamate through vesicular exocytosis to mediate neuron-glial interactions. In contrast to exocytosis, the endocytic pathways in astroglial cells are poorly understood. Here, we identify a constitutive endocytic pathway in cultured astrocytes that is dependent on neither clathrin nor dynamin. This dynamin-independent endocytic pathway is regulated by Rab5, an early endosome protein. The endocytosed vesicles show fast transition from early endosomes to late endosomes and lysosomes within a few minutes. Interestingly, this clathrin-and dynamin-independent endocytosis in astrocytes is potently regulated by intracellular Ca2+. ATP and glutamate greatly enhance the dynamin-independent endocytosis through elevating the intracellular Ca2+. In addition, amyloid-β peptide (Aβ) also enhances the dynamin-independent endocytosis by inducing Ca2+ transients in astrocytes. These results demonstrate a novel endocytic pathway in glial cells that is dynamin independent but tightly regulated by intracellular Ca2+. The regulation by ATP, glutamate, and Aβ suggests an important role of the dynamin-independent endocytosis in both physiological and pathological conditions.
AB - Astrocytes release ATP and glutamate through vesicular exocytosis to mediate neuron-glial interactions. In contrast to exocytosis, the endocytic pathways in astroglial cells are poorly understood. Here, we identify a constitutive endocytic pathway in cultured astrocytes that is dependent on neither clathrin nor dynamin. This dynamin-independent endocytic pathway is regulated by Rab5, an early endosome protein. The endocytosed vesicles show fast transition from early endosomes to late endosomes and lysosomes within a few minutes. Interestingly, this clathrin-and dynamin-independent endocytosis in astrocytes is potently regulated by intracellular Ca2+. ATP and glutamate greatly enhance the dynamin-independent endocytosis through elevating the intracellular Ca2+. In addition, amyloid-β peptide (Aβ) also enhances the dynamin-independent endocytosis by inducing Ca2+ transients in astrocytes. These results demonstrate a novel endocytic pathway in glial cells that is dynamin independent but tightly regulated by intracellular Ca2+. The regulation by ATP, glutamate, and Aβ suggests an important role of the dynamin-independent endocytosis in both physiological and pathological conditions.
UR - http://www.scopus.com/inward/record.url?scp=67649371201&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67649371201&partnerID=8YFLogxK
U2 - 10.1523/JNEUROSCI.6139-08.2009
DO - 10.1523/JNEUROSCI.6139-08.2009
M3 - Article
C2 - 19553446
AN - SCOPUS:67649371201
VL - 29
SP - 8063
EP - 8074
JO - Journal of Neuroscience
JF - Journal of Neuroscience
SN - 0270-6474
IS - 25
ER -