Ca 2+ regulation of dynamin-independent endocytosis in cortical astrocytes

Min Jiang, Gong Chen

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Astrocytes release ATP and glutamate through vesicular exocytosis to mediate neuron-glial interactions. In contrast to exocytosis, the endocytic pathways in astroglial cells are poorly understood. Here, we identify a constitutive endocytic pathway in cultured astrocytes that is dependent on neither clathrin nor dynamin. This dynamin-independent endocytic pathway is regulated by Rab5, an early endosome protein. The endocytosed vesicles show fast transition from early endosomes to late endosomes and lysosomes within a few minutes. Interestingly, this clathrin-and dynamin-independent endocytosis in astrocytes is potently regulated by intracellular Ca 2+ . ATP and glutamate greatly enhance the dynamin-independent endocytosis through elevating the intracellular Ca 2+ . In addition, amyloid-β peptide (Aβ) also enhances the dynamin-independent endocytosis by inducing Ca 2+ transients in astrocytes. These results demonstrate a novel endocytic pathway in glial cells that is dynamin independent but tightly regulated by intracellular Ca 2+ . The regulation by ATP, glutamate, and Aβ suggests an important role of the dynamin-independent endocytosis in both physiological and pathological conditions.

Original languageEnglish (US)
Pages (from-to)8063-8074
Number of pages12
JournalJournal of Neuroscience
Volume29
Issue number25
DOIs
StatePublished - Jun 24 2009

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Dynamin II
Dynamins
Endocytosis
Astrocytes
Endosomes
Glutamic Acid
Clathrin
Adenosine Triphosphate
Exocytosis
Neuroglia
Lysosomes
Amyloid
Neurons

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

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abstract = "Astrocytes release ATP and glutamate through vesicular exocytosis to mediate neuron-glial interactions. In contrast to exocytosis, the endocytic pathways in astroglial cells are poorly understood. Here, we identify a constitutive endocytic pathway in cultured astrocytes that is dependent on neither clathrin nor dynamin. This dynamin-independent endocytic pathway is regulated by Rab5, an early endosome protein. The endocytosed vesicles show fast transition from early endosomes to late endosomes and lysosomes within a few minutes. Interestingly, this clathrin-and dynamin-independent endocytosis in astrocytes is potently regulated by intracellular Ca 2+ . ATP and glutamate greatly enhance the dynamin-independent endocytosis through elevating the intracellular Ca 2+ . In addition, amyloid-β peptide (Aβ) also enhances the dynamin-independent endocytosis by inducing Ca 2+ transients in astrocytes. These results demonstrate a novel endocytic pathway in glial cells that is dynamin independent but tightly regulated by intracellular Ca 2+ . The regulation by ATP, glutamate, and Aβ suggests an important role of the dynamin-independent endocytosis in both physiological and pathological conditions.",
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Ca 2+ regulation of dynamin-independent endocytosis in cortical astrocytes . / Jiang, Min; Chen, Gong.

In: Journal of Neuroscience, Vol. 29, No. 25, 24.06.2009, p. 8063-8074.

Research output: Contribution to journalArticle

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AU - Chen, Gong

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AB - Astrocytes release ATP and glutamate through vesicular exocytosis to mediate neuron-glial interactions. In contrast to exocytosis, the endocytic pathways in astroglial cells are poorly understood. Here, we identify a constitutive endocytic pathway in cultured astrocytes that is dependent on neither clathrin nor dynamin. This dynamin-independent endocytic pathway is regulated by Rab5, an early endosome protein. The endocytosed vesicles show fast transition from early endosomes to late endosomes and lysosomes within a few minutes. Interestingly, this clathrin-and dynamin-independent endocytosis in astrocytes is potently regulated by intracellular Ca 2+ . ATP and glutamate greatly enhance the dynamin-independent endocytosis through elevating the intracellular Ca 2+ . In addition, amyloid-β peptide (Aβ) also enhances the dynamin-independent endocytosis by inducing Ca 2+ transients in astrocytes. These results demonstrate a novel endocytic pathway in glial cells that is dynamin independent but tightly regulated by intracellular Ca 2+ . The regulation by ATP, glutamate, and Aβ suggests an important role of the dynamin-independent endocytosis in both physiological and pathological conditions.

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