Calcitonin is expressed in the chicken pituitary gland: Influence of gonadal steroids and sexual maturation

Sreenivasa R. Maddineni, Susan M. Krzysik-Walker, Olga M. Ocón-Grove, Susan Marie Perrine, Gilbert L. Hendricks, Ramesh Ramachandran

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Calcitonin (CT) is primarily produced by the thyroid C cells in mammals or by the ultimobranchial gland in chickens. CT is also expressed by the pituitary gland in rats in which it functions as a paracrine factor causing decreased lactotroph proliferation and prolactin (PRL) secretion. Gonadal steroids influence CT expression in the rat pituitary gland. However, the expression of the CT gene in the pituitary gland of chickens or of any other avian species has not previously been reported. We have tested the hypotheses that CT is expressed in the chicken pituitary gland, and that its expression is influenced by sexual maturation or in response to ovarian steroid administration. We have detected robust expression of CT cDNA in the chicken pituitary gland by reverse transcription/polymerase chain reaction (PCR). The sequence of the pituitary-derived CT cDNA is identical to that of the ultimobranchial gland. CT-immunoreactive (ir) cells have been observed throughout the anterior pituitary gland by confocal microscopy. Many of the PRL-ir cells show co-localization with CT-ir cells. Quantitative real-time PCR analysis has revealed an inverse relationship between the quantities of PRL mRNA and CT mRNA in the pituitary gland: sexually mature hens contain lower amounts of CT mRNA but larger quantities of PRL mRNA compared with sexually immature chickens. Estradiol and/or progesterone treatment of sexually immature chickens leads to a significant decrease in the quantity of pituitary CT mRNA relative to that in the vehicle-treated chickens. We conclude that pituitary CT plays an important paracrine/autocrine role in the control of lactotroph function and PRL secretion in the chicken.

Original languageEnglish (US)
Pages (from-to)521-528
Number of pages8
JournalCell And Tissue Research
Volume327
Issue number3
DOIs
StatePublished - Mar 1 2007

Fingerprint

Sexual Maturation
Calcitonin
Pituitary Gland
Chickens
Steroids
Prolactin
Messenger RNA
Lactotrophs
Polymerase chain reaction
Rats
Complementary DNA
Anterior Pituitary Gland
Mammals
Confocal microscopy
Transcription
Confocal Microscopy
Reverse Transcription
Progesterone
Real-Time Polymerase Chain Reaction
Estradiol

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Clinical Biochemistry
  • Anatomy

Cite this

Maddineni, Sreenivasa R. ; Krzysik-Walker, Susan M. ; Ocón-Grove, Olga M. ; Perrine, Susan Marie ; Hendricks, Gilbert L. ; Ramachandran, Ramesh. / Calcitonin is expressed in the chicken pituitary gland : Influence of gonadal steroids and sexual maturation. In: Cell And Tissue Research. 2007 ; Vol. 327, No. 3. pp. 521-528.
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Calcitonin is expressed in the chicken pituitary gland : Influence of gonadal steroids and sexual maturation. / Maddineni, Sreenivasa R.; Krzysik-Walker, Susan M.; Ocón-Grove, Olga M.; Perrine, Susan Marie; Hendricks, Gilbert L.; Ramachandran, Ramesh.

In: Cell And Tissue Research, Vol. 327, No. 3, 01.03.2007, p. 521-528.

Research output: Contribution to journalArticle

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T1 - Calcitonin is expressed in the chicken pituitary gland

T2 - Influence of gonadal steroids and sexual maturation

AU - Maddineni, Sreenivasa R.

AU - Krzysik-Walker, Susan M.

AU - Ocón-Grove, Olga M.

AU - Perrine, Susan Marie

AU - Hendricks, Gilbert L.

AU - Ramachandran, Ramesh

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N2 - Calcitonin (CT) is primarily produced by the thyroid C cells in mammals or by the ultimobranchial gland in chickens. CT is also expressed by the pituitary gland in rats in which it functions as a paracrine factor causing decreased lactotroph proliferation and prolactin (PRL) secretion. Gonadal steroids influence CT expression in the rat pituitary gland. However, the expression of the CT gene in the pituitary gland of chickens or of any other avian species has not previously been reported. We have tested the hypotheses that CT is expressed in the chicken pituitary gland, and that its expression is influenced by sexual maturation or in response to ovarian steroid administration. We have detected robust expression of CT cDNA in the chicken pituitary gland by reverse transcription/polymerase chain reaction (PCR). The sequence of the pituitary-derived CT cDNA is identical to that of the ultimobranchial gland. CT-immunoreactive (ir) cells have been observed throughout the anterior pituitary gland by confocal microscopy. Many of the PRL-ir cells show co-localization with CT-ir cells. Quantitative real-time PCR analysis has revealed an inverse relationship between the quantities of PRL mRNA and CT mRNA in the pituitary gland: sexually mature hens contain lower amounts of CT mRNA but larger quantities of PRL mRNA compared with sexually immature chickens. Estradiol and/or progesterone treatment of sexually immature chickens leads to a significant decrease in the quantity of pituitary CT mRNA relative to that in the vehicle-treated chickens. We conclude that pituitary CT plays an important paracrine/autocrine role in the control of lactotroph function and PRL secretion in the chicken.

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