Calcium regulates the commitment of murine erythroleukemia cells to terminal erythroid differentiation.

K. Bridges, Robert Levenson, D. Housman, L. Cantley

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

An alteration in the rate of calcium transport appears to be the rate-limiting event for the commitment of murine erythroleukemia (MEL) cells to initiate a program of terminal erythroid differentiation. The dimethyl sulfoxide (DMSO)-induced commitment of MEL cells to erythroid differentiation can be inhibited by treatment of cells with the calcium-chelating agent EGTA. Upon removal of EGTA, cells initiate commitment without the 12-h lag normally observed after treatment with DMSO alone. Treatment of cells with DMSO in the presence of calcium ionophore A23187 causes cells to initiate commitment from time zero with no lag. These results suggest that the lag is the time required for DMSO to alter the calcium transport properties of the cell.

Original languageEnglish (US)
Pages (from-to)542-544
Number of pages3
JournalThe Journal of cell biology
Volume90
Issue number2
DOIs
StatePublished - Jan 1 1981

Fingerprint

Leukemia, Erythroblastic, Acute
Dimethyl Sulfoxide
Calcium
Egtazic Acid
Erythroid Cells
Calcium Ionophores
Calcimycin

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

@article{670c82f5de1e46198fffa78111593093,
title = "Calcium regulates the commitment of murine erythroleukemia cells to terminal erythroid differentiation.",
abstract = "An alteration in the rate of calcium transport appears to be the rate-limiting event for the commitment of murine erythroleukemia (MEL) cells to initiate a program of terminal erythroid differentiation. The dimethyl sulfoxide (DMSO)-induced commitment of MEL cells to erythroid differentiation can be inhibited by treatment of cells with the calcium-chelating agent EGTA. Upon removal of EGTA, cells initiate commitment without the 12-h lag normally observed after treatment with DMSO alone. Treatment of cells with DMSO in the presence of calcium ionophore A23187 causes cells to initiate commitment from time zero with no lag. These results suggest that the lag is the time required for DMSO to alter the calcium transport properties of the cell.",
author = "K. Bridges and Robert Levenson and D. Housman and L. Cantley",
year = "1981",
month = "1",
day = "1",
doi = "10.1083/jcb.90.2.542",
language = "English (US)",
volume = "90",
pages = "542--544",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "2",

}

Calcium regulates the commitment of murine erythroleukemia cells to terminal erythroid differentiation. / Bridges, K.; Levenson, Robert; Housman, D.; Cantley, L.

In: The Journal of cell biology, Vol. 90, No. 2, 01.01.1981, p. 542-544.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Calcium regulates the commitment of murine erythroleukemia cells to terminal erythroid differentiation.

AU - Bridges, K.

AU - Levenson, Robert

AU - Housman, D.

AU - Cantley, L.

PY - 1981/1/1

Y1 - 1981/1/1

N2 - An alteration in the rate of calcium transport appears to be the rate-limiting event for the commitment of murine erythroleukemia (MEL) cells to initiate a program of terminal erythroid differentiation. The dimethyl sulfoxide (DMSO)-induced commitment of MEL cells to erythroid differentiation can be inhibited by treatment of cells with the calcium-chelating agent EGTA. Upon removal of EGTA, cells initiate commitment without the 12-h lag normally observed after treatment with DMSO alone. Treatment of cells with DMSO in the presence of calcium ionophore A23187 causes cells to initiate commitment from time zero with no lag. These results suggest that the lag is the time required for DMSO to alter the calcium transport properties of the cell.

AB - An alteration in the rate of calcium transport appears to be the rate-limiting event for the commitment of murine erythroleukemia (MEL) cells to initiate a program of terminal erythroid differentiation. The dimethyl sulfoxide (DMSO)-induced commitment of MEL cells to erythroid differentiation can be inhibited by treatment of cells with the calcium-chelating agent EGTA. Upon removal of EGTA, cells initiate commitment without the 12-h lag normally observed after treatment with DMSO alone. Treatment of cells with DMSO in the presence of calcium ionophore A23187 causes cells to initiate commitment from time zero with no lag. These results suggest that the lag is the time required for DMSO to alter the calcium transport properties of the cell.

UR - http://www.scopus.com/inward/record.url?scp=0019598750&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019598750&partnerID=8YFLogxK

U2 - 10.1083/jcb.90.2.542

DO - 10.1083/jcb.90.2.542

M3 - Article

VL - 90

SP - 542

EP - 544

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 2

ER -