Calcium-sensing mechanism in TRPC5 channels contributing to retardation of neurite outgrowth

Hui Hui, Damian McHugh, Meredith Hannan, Fanning Zeng, Shang Zhong Xu, Saeed ul Hassan Khan, Robert Levenson, David J. Beech, Jamie L. Weiss

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

The calcium- and sodium-permeable transient receptor potential channel TRPC5 has an inhibitory role in neuronal outgrowth but the mechanisms governing its activity are poorly understood. Here we propose a mechanism involving the neuronal calcium sensor-1 (NCS-1) protein. Inhibitory mutants of TRPC5 and NCS-1 enhance neurite outgrowth similarly. Mutant NCS-1 does not inhibit surface-expression of TRPC5 but generally suppresses channel activity, irrespective of whether it is evoked by carbachol, store depletion, lanthanides or elevated intracellular calcium. NCS-1 and TRPC5 are in the same protein complex in rat brain and NCS-1 directly binds to the TRPC5 C-terminus. The data suggest protein-protein interaction between NCS-1 and TRPC5, and involvement of this protein complex in retardation of neurite outgrowth.

Original languageEnglish (US)
Pages (from-to)165-172
Number of pages8
JournalJournal of Physiology
Volume572
Issue number1
DOIs
StatePublished - Apr 1 2006

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Calcium
Neuronal Calcium-Sensor Proteins
Proteins
Transient Receptor Potential Channels
Lanthanoid Series Elements
Carbachol
frequenin calcium sensor proteins
Neuronal Outgrowth
Sodium
Brain

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

Hui, H., McHugh, D., Hannan, M., Zeng, F., Xu, S. Z., Khan, S. U. H., ... Weiss, J. L. (2006). Calcium-sensing mechanism in TRPC5 channels contributing to retardation of neurite outgrowth. Journal of Physiology, 572(1), 165-172. https://doi.org/10.1113/jphysiol.2005.102889
Hui, Hui ; McHugh, Damian ; Hannan, Meredith ; Zeng, Fanning ; Xu, Shang Zhong ; Khan, Saeed ul Hassan ; Levenson, Robert ; Beech, David J. ; Weiss, Jamie L. / Calcium-sensing mechanism in TRPC5 channels contributing to retardation of neurite outgrowth. In: Journal of Physiology. 2006 ; Vol. 572, No. 1. pp. 165-172.
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Hui, H, McHugh, D, Hannan, M, Zeng, F, Xu, SZ, Khan, SUH, Levenson, R, Beech, DJ & Weiss, JL 2006, 'Calcium-sensing mechanism in TRPC5 channels contributing to retardation of neurite outgrowth', Journal of Physiology, vol. 572, no. 1, pp. 165-172. https://doi.org/10.1113/jphysiol.2005.102889

Calcium-sensing mechanism in TRPC5 channels contributing to retardation of neurite outgrowth. / Hui, Hui; McHugh, Damian; Hannan, Meredith; Zeng, Fanning; Xu, Shang Zhong; Khan, Saeed ul Hassan; Levenson, Robert; Beech, David J.; Weiss, Jamie L.

In: Journal of Physiology, Vol. 572, No. 1, 01.04.2006, p. 165-172.

Research output: Contribution to journalArticle

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AU - Hui, Hui

AU - McHugh, Damian

AU - Hannan, Meredith

AU - Zeng, Fanning

AU - Xu, Shang Zhong

AU - Khan, Saeed ul Hassan

AU - Levenson, Robert

AU - Beech, David J.

AU - Weiss, Jamie L.

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AB - The calcium- and sodium-permeable transient receptor potential channel TRPC5 has an inhibitory role in neuronal outgrowth but the mechanisms governing its activity are poorly understood. Here we propose a mechanism involving the neuronal calcium sensor-1 (NCS-1) protein. Inhibitory mutants of TRPC5 and NCS-1 enhance neurite outgrowth similarly. Mutant NCS-1 does not inhibit surface-expression of TRPC5 but generally suppresses channel activity, irrespective of whether it is evoked by carbachol, store depletion, lanthanides or elevated intracellular calcium. NCS-1 and TRPC5 are in the same protein complex in rat brain and NCS-1 directly binds to the TRPC5 C-terminus. The data suggest protein-protein interaction between NCS-1 and TRPC5, and involvement of this protein complex in retardation of neurite outgrowth.

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