Calculation of serum "total lipid" concentrations for the adjustment of persistent organohalogen toxicant measurements in human samples

John T. Bernert, Wayman E. Turner, Donald George Jr Patterson, Larry L. Needham

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

Persistent organohalogen toxicants such as 2,3,7,8-tetrachlorodibenzo-p-dioxin or polychlorinated biphenyls measured in human serum are often expressed on a lipid weight basis, most commonly by dividing the toxicants' concentration by the weight of total lipids in the sample. Therefore, the manner in which this lipid adjustment is calculated may influence the final reported result. Gravimetric total lipid assays have been used, but they are time-consuming and sometimes may be ill-defined. Consequently, alternative methods using enzymatic assays have been developed based on summing the individual lipid species measured. Recent reports, however, have suggested that significantly different total lipid results may be obtained when using alternative formulae in a summation approach. In this report, we summarize the results obtained from lipid measurements of nearly 900 samples made as part of a study of a group of older American men (mean age 62 years), and we compare our total lipid estimates obtained by using both our standard and "short" formula (the latter based on total cholesterol and triglycerides only) with results obtained using the recently proposed alternative formulae. Our findings indicate that both our long and short formulae provide similar estimates of serum total lipid concentrations, and that differences observed in lipid estimates when using the newer alternative summation methods may reflect differences in how the term "total lipid" is defined, especially with regard to the need to include the contribution of the weight of the cholesterol ester fatty acids in the calculation.

Original languageEnglish (US)
Pages (from-to)824-831
Number of pages8
JournalChemosphere
Volume68
Issue number5
DOIs
StatePublished - Jun 1 2007

Fingerprint

organohalogen
Lipids
serum
lipid
Serum
Cholesterol
Weights and Measures
Assays
calculation
assay
Cholesterol Esters
Polychlorinated Biphenyls
Enzyme Assays
Polychlorinated biphenyls
Fatty acids
dioxin
ester
PCB
Esters
Triglycerides

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

Bernert, John T. ; Turner, Wayman E. ; Patterson, Donald George Jr ; Needham, Larry L. / Calculation of serum "total lipid" concentrations for the adjustment of persistent organohalogen toxicant measurements in human samples. In: Chemosphere. 2007 ; Vol. 68, No. 5. pp. 824-831.
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Calculation of serum "total lipid" concentrations for the adjustment of persistent organohalogen toxicant measurements in human samples. / Bernert, John T.; Turner, Wayman E.; Patterson, Donald George Jr; Needham, Larry L.

In: Chemosphere, Vol. 68, No. 5, 01.06.2007, p. 824-831.

Research output: Contribution to journalArticle

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