Calorimetric sensing system for real-time urea and creatinine measurements

Son Vu Hoang Lai, Srinivas Tadigadapa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

A high-sensitivity, calorimetric, point-of-care device for automated bedside urine testing based on a micromachined thermal sensor is presented. Immobilized urease and creatinine iminohydrolase enzymes on thin (25 μm) Kapton® films are used to measure the catalytic calorimetric output as a function of concentration. The sensor design locates the active Kapton® film in close proximity to the quartz temperature sensor thereby providing an efficient heat coupling between the two. Measurements of urea concentration at room temperature using the continuous flow set-up and creatinine in open chamber setup at 37 °C are presented. Concentrations of 50 mM for urea and 100 μM for creatinine have been measured. The paper presents the first non-contact, continuous flow thermal sensing measurements. The unique sensor design allows for enzyme immobilized Kapton® film as an easy to swap disposable cartridge and represents a significant advancement for clinical diagnostic application.

Original languageEnglish (US)
Title of host publicationIEEE SENSORS 2012 - Proceedings
DOIs
StatePublished - Dec 1 2012
Event11th IEEE SENSORS 2012 Conference - Taipei, Taiwan, Province of China
Duration: Oct 28 2012Oct 31 2012

Publication series

NameProceedings of IEEE Sensors

Other

Other11th IEEE SENSORS 2012 Conference
CountryTaiwan, Province of China
CityTaipei
Period10/28/1210/31/12

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All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

Cite this

Lai, S. V. H., & Tadigadapa, S. (2012). Calorimetric sensing system for real-time urea and creatinine measurements. In IEEE SENSORS 2012 - Proceedings [6411359] (Proceedings of IEEE Sensors). https://doi.org/10.1109/ICSENS.2012.6411359