Capturing RNA-dependent pathways for cryo-EM analysis

Justin R. Tanner, Katherine Degen, Brian L. Gilmore, Deborah F. Kelly

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Cryo-Electron Microscopy (EM) is a powerful technique to visualize biological processes at nanometer resolution. Structural studies of macromolecular assemblies are typically performed on individual complexes that are biochemically isolated from their cellular context. Here we present a molecular imaging platform to capture and view multiple components of cellular pathways within a functionally relevant framework. We utilized the bacterial protein synthesis machinery as a model system to develop our approach. By using modified Affinity Grid surfaces, we were able to recruit multiple protein assemblies bound to nascent strands of mRNA. The combined use of Affinity Capture technology and single particle electron microscopy provide the basis for visualizing RNA-dependent pathways in a remarkable new way.

Original languageEnglish (US)
Article numbera7
Pages (from-to)e201204003
JournalComputational and Structural Biotechnology Journal
Volume1
Issue number1
DOIs
StatePublished - Apr 2012

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Computer Science Applications

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