Carbohydrate allocation patterns in citrus genotypes as affected by phosphorus nutrition, mycorrhizal colonization and mycorrhizal dependency

J. H. Graham, L. W. Duncan, David Eissenstat

Research output: Contribution to journalArticle

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Abstract

Among closely related citrus genotypes growing in high phosphorus (P) orchard soils, there is a tendency for less mycorrhizal-dependent (M-dependent) species to have lower rates of root colonization than more M-dependent species. We hypothesized that the less M-dependent the citrus species the more limited is their carbohydrate (CHO) allocation to the M fungus. In a glasshouse study at low and high P supply, lower total incidence of Glomus intraradices FL 208, intensity of vesicles formation and accumulation of 16: 1W5 cis fungal fatty acid as measures of root colonization were related to lower mycorrhizal dependency (MD; M plant d. wt/non-mycorrhizal (NM) plant d. wt expressed at low P supply) in five citrus genotypes. At high P supply, when host carbon (C) production was not affected by P nutrition, less M-dependent genotypes had consistently lower starch concentrations in their root and shoot tissues than did more M-dependent genotypes, irrespective of M inoculation. At low P supply, M plants were more heavily colonized by G. intraradices and had lower starch levels than the M plants supplied with additional P. At high P, M plants of the more dependent genotypes allocated more C to starch pools relative to the NM plant than the least dependent genotype. Concentration of sucrose in tissues did not vary consistently with the dependency of citrus genotypes or with M inoculation except in high P NM plants, when less M-dependent genotypes had lower levels of sucrose in their roots than the more dependent species. At high P supply, sucrose concentrations were lower in colonized roots than in NM roots. Across citrus genotypes, sucrose in M fibrous roots decreased relative to that in NM roots with increase in root colonization suggesting that more sucrose was allocated for growth and maintenance of G. intraradices in roots of M-dependent species. The concentration of reducing sugars in root tissues varied in relation to MD of citrus genotypes in the same way as that of starch and sucrose, but was less responsive to M colonization. Responses of total non-structural CHO in tissues indicated that more heavily colonized plants at low P expended more C to acquire P than did M plants of similar biomass and P status grown at high P supply. Total CHO pools increased with MD of citrus genotypes, providing evidence than C allocation patterns in the host affect M colonization.

Original languageEnglish (US)
Pages (from-to)335-343
Number of pages9
JournalNew Phytologist
Volume135
Issue number2
DOIs
StatePublished - Dec 1 1997

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Citrus
Phosphorus
Genotype
Carbohydrates
nutrition
carbohydrates
phosphorus
genotype
Sucrose
sucrose
Starch
Glomus intraradices
starch
fibrous roots
orchard soils
reducing sugars
Biomass
Fungi
Fatty Acids
Soil

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Cite this

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title = "Carbohydrate allocation patterns in citrus genotypes as affected by phosphorus nutrition, mycorrhizal colonization and mycorrhizal dependency",
abstract = "Among closely related citrus genotypes growing in high phosphorus (P) orchard soils, there is a tendency for less mycorrhizal-dependent (M-dependent) species to have lower rates of root colonization than more M-dependent species. We hypothesized that the less M-dependent the citrus species the more limited is their carbohydrate (CHO) allocation to the M fungus. In a glasshouse study at low and high P supply, lower total incidence of Glomus intraradices FL 208, intensity of vesicles formation and accumulation of 16: 1W5 cis fungal fatty acid as measures of root colonization were related to lower mycorrhizal dependency (MD; M plant d. wt/non-mycorrhizal (NM) plant d. wt expressed at low P supply) in five citrus genotypes. At high P supply, when host carbon (C) production was not affected by P nutrition, less M-dependent genotypes had consistently lower starch concentrations in their root and shoot tissues than did more M-dependent genotypes, irrespective of M inoculation. At low P supply, M plants were more heavily colonized by G. intraradices and had lower starch levels than the M plants supplied with additional P. At high P, M plants of the more dependent genotypes allocated more C to starch pools relative to the NM plant than the least dependent genotype. Concentration of sucrose in tissues did not vary consistently with the dependency of citrus genotypes or with M inoculation except in high P NM plants, when less M-dependent genotypes had lower levels of sucrose in their roots than the more dependent species. At high P supply, sucrose concentrations were lower in colonized roots than in NM roots. Across citrus genotypes, sucrose in M fibrous roots decreased relative to that in NM roots with increase in root colonization suggesting that more sucrose was allocated for growth and maintenance of G. intraradices in roots of M-dependent species. The concentration of reducing sugars in root tissues varied in relation to MD of citrus genotypes in the same way as that of starch and sucrose, but was less responsive to M colonization. Responses of total non-structural CHO in tissues indicated that more heavily colonized plants at low P expended more C to acquire P than did M plants of similar biomass and P status grown at high P supply. Total CHO pools increased with MD of citrus genotypes, providing evidence than C allocation patterns in the host affect M colonization.",
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Carbohydrate allocation patterns in citrus genotypes as affected by phosphorus nutrition, mycorrhizal colonization and mycorrhizal dependency. / Graham, J. H.; Duncan, L. W.; Eissenstat, David.

In: New Phytologist, Vol. 135, No. 2, 01.12.1997, p. 335-343.

Research output: Contribution to journalArticle

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AB - Among closely related citrus genotypes growing in high phosphorus (P) orchard soils, there is a tendency for less mycorrhizal-dependent (M-dependent) species to have lower rates of root colonization than more M-dependent species. We hypothesized that the less M-dependent the citrus species the more limited is their carbohydrate (CHO) allocation to the M fungus. In a glasshouse study at low and high P supply, lower total incidence of Glomus intraradices FL 208, intensity of vesicles formation and accumulation of 16: 1W5 cis fungal fatty acid as measures of root colonization were related to lower mycorrhizal dependency (MD; M plant d. wt/non-mycorrhizal (NM) plant d. wt expressed at low P supply) in five citrus genotypes. At high P supply, when host carbon (C) production was not affected by P nutrition, less M-dependent genotypes had consistently lower starch concentrations in their root and shoot tissues than did more M-dependent genotypes, irrespective of M inoculation. At low P supply, M plants were more heavily colonized by G. intraradices and had lower starch levels than the M plants supplied with additional P. At high P, M plants of the more dependent genotypes allocated more C to starch pools relative to the NM plant than the least dependent genotype. Concentration of sucrose in tissues did not vary consistently with the dependency of citrus genotypes or with M inoculation except in high P NM plants, when less M-dependent genotypes had lower levels of sucrose in their roots than the more dependent species. At high P supply, sucrose concentrations were lower in colonized roots than in NM roots. Across citrus genotypes, sucrose in M fibrous roots decreased relative to that in NM roots with increase in root colonization suggesting that more sucrose was allocated for growth and maintenance of G. intraradices in roots of M-dependent species. The concentration of reducing sugars in root tissues varied in relation to MD of citrus genotypes in the same way as that of starch and sucrose, but was less responsive to M colonization. Responses of total non-structural CHO in tissues indicated that more heavily colonized plants at low P expended more C to acquire P than did M plants of similar biomass and P status grown at high P supply. Total CHO pools increased with MD of citrus genotypes, providing evidence than C allocation patterns in the host affect M colonization.

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