Carbon-detected 15N NMR spin relaxation of an intrinsically disordered protein: FCP1 dynamics unbound and in complex with RAP74

Chad W. Lawrence, Scott A. Showalter

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Intrinsically disordered proteins (IDPs) lack unique 3D structures under native conditions and as such exist as highly dynamic ensembles in solution. We present two 13C-direct detection experiments for the measurement of 15N NMR spin relaxation called the CON(T1)-IPAP and CON(T2)-IPAP that quantify backbone dynamics on a per-residue basis for IDPs in solution. These experiments have been applied to the intrinsically disordered C-terminal of FCP1, both free in solution and while bound to the RAP74 winged-helix domain. The results provide evidence that most of FCP1 remains highly dynamic in both states, while the 20 residues forming direct contact with RAP74 become more ordered in the complex. Parallel analysis of RAP74 backbone 15N NMR spin relaxation reveals only very limited ordering of RAP74 upon FCP1 binding. Taken together, these data show that folding-upon-binding is highly local in this system, with disorder prevailing even in the complex.

Original languageEnglish (US)
Pages (from-to)1409-1413
Number of pages5
JournalJournal of Physical Chemistry Letters
Volume3
Issue number10
DOIs
StatePublished - May 17 2012

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Physical and Theoretical Chemistry

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