Carbon, nitrogen and pH regulate the production and activity of a polygalacturonase isozyme produced by Penicillium expansum

Wayne M. Jurick, Ivana Vico, Verneta L. Gaskins, Kari Anne Peter, Eunhee Park, Wojciech J. Janisiewicz, William S. Conway

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The influence of carbon, nitrogen and pH on polygalacturonase (PG) activity produced by Penicillium expansum were investigated. P. expansum mycelial growth was greatest on lyophilized lyophilised fruit tissue and the highest PG activity occurred in apple pectin medium. Nitrogen source influenced PG activity and was highest with ammonia while the greatest mycelial mass was supported by glutamate or glutamine. PG activity and mycelial mass peaked 5 five days after inoculation as polyuronide content decreased and the pH and ammonium levels increased in apple pectin medium. A single active PG isozyme with an isoelectric point of ~7.6 was produced in apple pectin medium and a partial cDNA clone was obtained that was most homologous to the pggII gene from Penicillium. griseoroseum. The results from this study indicate that P. expansum can modulate the activity of PG in response to nutrient sources and ambient pH through signalling pathways that modulate nutrient acquisition, uptake and metabolism.

Original languageEnglish (US)
Pages (from-to)1101-1114
Number of pages14
JournalArchives of Phytopathology and Plant Protection
Volume45
Issue number9
DOIs
StatePublished - May 1 2012

Fingerprint

Penicillium expansum
polygalacturonase
isozymes
carbon
nitrogen
pectins
apples
nutrients
isoelectric point
Penicillium
glutamine
glutamates
ammonia
clones
uptake mechanisms
metabolism
fruits
genes

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science

Cite this

Jurick, Wayne M. ; Vico, Ivana ; Gaskins, Verneta L. ; Peter, Kari Anne ; Park, Eunhee ; Janisiewicz, Wojciech J. ; Conway, William S. / Carbon, nitrogen and pH regulate the production and activity of a polygalacturonase isozyme produced by Penicillium expansum. In: Archives of Phytopathology and Plant Protection. 2012 ; Vol. 45, No. 9. pp. 1101-1114.
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Carbon, nitrogen and pH regulate the production and activity of a polygalacturonase isozyme produced by Penicillium expansum. / Jurick, Wayne M.; Vico, Ivana; Gaskins, Verneta L.; Peter, Kari Anne; Park, Eunhee; Janisiewicz, Wojciech J.; Conway, William S.

In: Archives of Phytopathology and Plant Protection, Vol. 45, No. 9, 01.05.2012, p. 1101-1114.

Research output: Contribution to journalArticle

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