Carboxymethyldextran Lactone: A Preactivated Polymer for Amine Conjugations

Ned D. Heindel, Michael A. Kauffman, Eric K. Akyea, Stephanie A. Engel, Michael F. Frey, C. Jeffrey Lacey, Roger Egolf

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The linking of amino haptens to carboxymethyldextran (CMD) requires carboxyl activation, for example, via carbodiimides. We have discovered that substantial N-acylurea, derived from these carbodiimides, can be trapped on the CMD backbone. As an alternative, CMD can be conveniently lactonized by heating in inert solvents, and this carboxymethyldextran lactone (CDL) can be employed directly for amine conjugation.

Original languageEnglish (US)
Pages (from-to)98-100
Number of pages3
JournalBioconjugate Chemistry
Volume5
Issue number1
DOIs
StatePublished - Jan 1 1994

Fingerprint

Lactones
Amines
Polymers
Chemical activation
Carbodiimides
Heating
Haptens
carboxymethyl dextran

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

Heindel, N. D., Kauffman, M. A., Akyea, E. K., Engel, S. A., Frey, M. F., Lacey, C. J., & Egolf, R. (1994). Carboxymethyldextran Lactone: A Preactivated Polymer for Amine Conjugations. Bioconjugate Chemistry, 5(1), 98-100. https://doi.org/10.1021/bc00025a013
Heindel, Ned D. ; Kauffman, Michael A. ; Akyea, Eric K. ; Engel, Stephanie A. ; Frey, Michael F. ; Lacey, C. Jeffrey ; Egolf, Roger. / Carboxymethyldextran Lactone : A Preactivated Polymer for Amine Conjugations. In: Bioconjugate Chemistry. 1994 ; Vol. 5, No. 1. pp. 98-100.
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Heindel, ND, Kauffman, MA, Akyea, EK, Engel, SA, Frey, MF, Lacey, CJ & Egolf, R 1994, 'Carboxymethyldextran Lactone: A Preactivated Polymer for Amine Conjugations', Bioconjugate Chemistry, vol. 5, no. 1, pp. 98-100. https://doi.org/10.1021/bc00025a013

Carboxymethyldextran Lactone : A Preactivated Polymer for Amine Conjugations. / Heindel, Ned D.; Kauffman, Michael A.; Akyea, Eric K.; Engel, Stephanie A.; Frey, Michael F.; Lacey, C. Jeffrey; Egolf, Roger.

In: Bioconjugate Chemistry, Vol. 5, No. 1, 01.01.1994, p. 98-100.

Research output: Contribution to journalArticle

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AU - Lacey, C. Jeffrey

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Heindel ND, Kauffman MA, Akyea EK, Engel SA, Frey MF, Lacey CJ et al. Carboxymethyldextran Lactone: A Preactivated Polymer for Amine Conjugations. Bioconjugate Chemistry. 1994 Jan 1;5(1):98-100. https://doi.org/10.1021/bc00025a013