The linking of amino haptens to carboxymethyldextran (CMD) requires carboxyl activation, for example, via carbodiimides. We have discovered that substantial N-acylurea, derived from these carbodiimides, can be trapped on the CMD backbone. As an alternative, CMD can be conveniently lactonized by heating in inert solvents, and this carboxymethyldextran lactone (CDL) can be employed directly for amine conjugation.
All Science Journal Classification (ASJC) codes
- Biomedical Engineering
- Pharmaceutical Science
- Organic Chemistry