Cardioplegia-induced cell swelling: Prevention by normothermic infusion

Edward Stephenson, A. Mark Jayawant, Clive M. Baumgarten, Ralph J. Damiano

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background. Previous work has shown significant swelling of isolated rabbit myocytes exposed to cold hyperkalemic cardioplegia; however, the effect of warm hyperkalemic cardioplegia on myocyte volume is unknown. This study examined the effect of warm hyperkalemic cardioplegia (St. Thomas' solution) on myocyte volume. Methods. Myocytes were enzymatically isolated and placed on an inverted video microscope. Tyrode's solution (37°C) was infused for 10 minutes to establish baseline cell volumes. Subsequently, either the control Tyrode's or St. Thomas' was infused either at 37°C and 9°C respectively (n = 5 for all groups) for 20 minutes, followed by a 30- minute reperfusion with 37°C Tyrode's. Cell volume was determined from cell images captured every 5 minutes. Results. Myocyte swelling occurred rapidly on exposure to cold St. Thomas' solution to a maximum of 9.8 ± 2.1% (p < 0.001). In contrast, myocytes exposed to warm cardioplegia did not show any volume changes during exposure to cardioplegia. However, upon reexposure to Tyrode's, these cells showed shrinkage below their baseline volume (p < 0.001). Conclusions. The cell swelling associated with hypothermic cardioplegia is prevented by normothermic infusion. (C) 2000 by The Society of Thoracic Surgeons.

Original languageEnglish (US)
Pages (from-to)1393-1398
Number of pages6
JournalAnnals of Thoracic Surgery
Volume69
Issue number5
DOIs
StatePublished - May 1 2000

Fingerprint

Induced Heart Arrest
Muscle Cells
Cell Size
Reperfusion
Rabbits

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

Stephenson, E., Mark Jayawant, A., Baumgarten, C. M., & Damiano, R. J. (2000). Cardioplegia-induced cell swelling: Prevention by normothermic infusion. Annals of Thoracic Surgery, 69(5), 1393-1398. https://doi.org/10.1016/S0003-4975(00)01227-3
Stephenson, Edward ; Mark Jayawant, A. ; Baumgarten, Clive M. ; Damiano, Ralph J. / Cardioplegia-induced cell swelling : Prevention by normothermic infusion. In: Annals of Thoracic Surgery. 2000 ; Vol. 69, No. 5. pp. 1393-1398.
@article{5f3622882d844f0398b4bd0faeb12ad9,
title = "Cardioplegia-induced cell swelling: Prevention by normothermic infusion",
abstract = "Background. Previous work has shown significant swelling of isolated rabbit myocytes exposed to cold hyperkalemic cardioplegia; however, the effect of warm hyperkalemic cardioplegia on myocyte volume is unknown. This study examined the effect of warm hyperkalemic cardioplegia (St. Thomas' solution) on myocyte volume. Methods. Myocytes were enzymatically isolated and placed on an inverted video microscope. Tyrode's solution (37°C) was infused for 10 minutes to establish baseline cell volumes. Subsequently, either the control Tyrode's or St. Thomas' was infused either at 37°C and 9°C respectively (n = 5 for all groups) for 20 minutes, followed by a 30- minute reperfusion with 37°C Tyrode's. Cell volume was determined from cell images captured every 5 minutes. Results. Myocyte swelling occurred rapidly on exposure to cold St. Thomas' solution to a maximum of 9.8 ± 2.1{\%} (p < 0.001). In contrast, myocytes exposed to warm cardioplegia did not show any volume changes during exposure to cardioplegia. However, upon reexposure to Tyrode's, these cells showed shrinkage below their baseline volume (p < 0.001). Conclusions. The cell swelling associated with hypothermic cardioplegia is prevented by normothermic infusion. (C) 2000 by The Society of Thoracic Surgeons.",
author = "Edward Stephenson and {Mark Jayawant}, A. and Baumgarten, {Clive M.} and Damiano, {Ralph J.}",
year = "2000",
month = "5",
day = "1",
doi = "10.1016/S0003-4975(00)01227-3",
language = "English (US)",
volume = "69",
pages = "1393--1398",
journal = "Annals of Thoracic Surgery",
issn = "0003-4975",
publisher = "Elsevier USA",
number = "5",

}

Stephenson, E, Mark Jayawant, A, Baumgarten, CM & Damiano, RJ 2000, 'Cardioplegia-induced cell swelling: Prevention by normothermic infusion', Annals of Thoracic Surgery, vol. 69, no. 5, pp. 1393-1398. https://doi.org/10.1016/S0003-4975(00)01227-3

Cardioplegia-induced cell swelling : Prevention by normothermic infusion. / Stephenson, Edward; Mark Jayawant, A.; Baumgarten, Clive M.; Damiano, Ralph J.

In: Annals of Thoracic Surgery, Vol. 69, No. 5, 01.05.2000, p. 1393-1398.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cardioplegia-induced cell swelling

T2 - Prevention by normothermic infusion

AU - Stephenson, Edward

AU - Mark Jayawant, A.

AU - Baumgarten, Clive M.

AU - Damiano, Ralph J.

PY - 2000/5/1

Y1 - 2000/5/1

N2 - Background. Previous work has shown significant swelling of isolated rabbit myocytes exposed to cold hyperkalemic cardioplegia; however, the effect of warm hyperkalemic cardioplegia on myocyte volume is unknown. This study examined the effect of warm hyperkalemic cardioplegia (St. Thomas' solution) on myocyte volume. Methods. Myocytes were enzymatically isolated and placed on an inverted video microscope. Tyrode's solution (37°C) was infused for 10 minutes to establish baseline cell volumes. Subsequently, either the control Tyrode's or St. Thomas' was infused either at 37°C and 9°C respectively (n = 5 for all groups) for 20 minutes, followed by a 30- minute reperfusion with 37°C Tyrode's. Cell volume was determined from cell images captured every 5 minutes. Results. Myocyte swelling occurred rapidly on exposure to cold St. Thomas' solution to a maximum of 9.8 ± 2.1% (p < 0.001). In contrast, myocytes exposed to warm cardioplegia did not show any volume changes during exposure to cardioplegia. However, upon reexposure to Tyrode's, these cells showed shrinkage below their baseline volume (p < 0.001). Conclusions. The cell swelling associated with hypothermic cardioplegia is prevented by normothermic infusion. (C) 2000 by The Society of Thoracic Surgeons.

AB - Background. Previous work has shown significant swelling of isolated rabbit myocytes exposed to cold hyperkalemic cardioplegia; however, the effect of warm hyperkalemic cardioplegia on myocyte volume is unknown. This study examined the effect of warm hyperkalemic cardioplegia (St. Thomas' solution) on myocyte volume. Methods. Myocytes were enzymatically isolated and placed on an inverted video microscope. Tyrode's solution (37°C) was infused for 10 minutes to establish baseline cell volumes. Subsequently, either the control Tyrode's or St. Thomas' was infused either at 37°C and 9°C respectively (n = 5 for all groups) for 20 minutes, followed by a 30- minute reperfusion with 37°C Tyrode's. Cell volume was determined from cell images captured every 5 minutes. Results. Myocyte swelling occurred rapidly on exposure to cold St. Thomas' solution to a maximum of 9.8 ± 2.1% (p < 0.001). In contrast, myocytes exposed to warm cardioplegia did not show any volume changes during exposure to cardioplegia. However, upon reexposure to Tyrode's, these cells showed shrinkage below their baseline volume (p < 0.001). Conclusions. The cell swelling associated with hypothermic cardioplegia is prevented by normothermic infusion. (C) 2000 by The Society of Thoracic Surgeons.

UR - http://www.scopus.com/inward/record.url?scp=0343517461&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0343517461&partnerID=8YFLogxK

U2 - 10.1016/S0003-4975(00)01227-3

DO - 10.1016/S0003-4975(00)01227-3

M3 - Article

C2 - 10881811

AN - SCOPUS:0343517461

VL - 69

SP - 1393

EP - 1398

JO - Annals of Thoracic Surgery

JF - Annals of Thoracic Surgery

SN - 0003-4975

IS - 5

ER -