Cargo ubiquitination is essential for multivesicular body intralumenal vesicle formation

Chris MacDonald, Nicholas J. Buchkovich, Daniel K. Stringer, Scott D. Emr, Robert C. Piper

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

The efficient formation of a variety of transport vesicles is influenced by the presence of cargo, suggesting that cargo itself might have a defining role in vesicle biogenesis. However, definitive in vivo experiments supporting this concept are lacking, as it is difficult to eliminate endogenous cargo. The Endosomal Sorting Complexes Required for Transport (ESCRT) apparatus sorts ubiquitinated membrane proteins into endosomal intralumenal vesicles (ILVs) that accumulate within multivesicular bodies. Here we show that cargo ubiquitination is required for effective recruitment of the ESCRT machinery onto endosomal membranes and for the subsequent formation of ILVs.

Original languageEnglish (US)
Pages (from-to)331-338
Number of pages8
JournalEMBO Reports
Volume13
Issue number4
DOIs
StatePublished - Apr 2012

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics

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