Carotenoid cleavage dioxygenase4 is a negative regulator of β-carotene content in arabidopsis seeds

Sabrina Gonzalez-Jorge, Sun Hwa Ha, Maria Magallanes-Lundback, Laura M. Ullrich, Ailing Zhou, Alexander E. Lipka, Yen Nhu Nguyen, Ruthie Angelovici, Haining Lin, Jason Cepela, Holly Little, C. Robin Buell, Michael A. Gore, Dean DellaPenna

Research output: Contribution to journalArticle

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Abstract

Experimental approaches targeting carotenoid biosynthetic enzymes have successfully increased the seed β-carotene content of crops. However, linkage analysis of seed carotenoids in Arabidopsis thaliana recombinant inbred populations showed that only 21% of quantitative trait loci, including those for β-carotene, encode carotenoid biosynthetic enzymes in their intervals. Thus, numerous loci remain uncharacterized and underutilized in biofortification approaches. Linkage mapping and genome-wide association studies of Arabidopsis seed carotenoids identified CAROTENOID CLEAVAGE DIOXYGENASE4 (CCD4) as a major negative regulator of seed carotenoid content, especially β-carotene. Loss of CCD4 function did not affect carotenoid homeostasis during seed development but greatly reduced carotenoid degradation during seed desiccation, increasing β-carotene content 8.4-fold relative to the wild type. Allelic complementation of a ccd4 null mutant demonstrated that single-nucleotide polymorphisms and insertions and deletions at the locus affect dry seed carotenoid content, due at least partly to differences in CCD4 expression. CCD4 also plays a major role in carotenoid turnover during dark-induced leaf senescence, with β-carotene accumulation again most strongly affected in the ccd4 mutant. These results demonstrate that CCD4 plays a major role in β-carotene degradation in drying seeds and senescing leaves and suggest that CCD4 orthologs would be promising targets for stabilizing and increasing the level of provitamin A carotenoids in seeds of major food crops.

Original languageEnglish (US)
Pages (from-to)4812-4826
Number of pages15
JournalPlant Cell
Volume25
Issue number12
DOIs
StatePublished - Dec 1 2013

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Carotenoids
carotenes
Arabidopsis
Seeds
carotenoids
seeds
biofortification
mutants
loci
degradation
food crops
desiccation (plant physiology)
enzymes
seed development
Desiccation
single nucleotide polymorphism
chromosome mapping
linkage (genetics)
Cotyledon
Chromosome Mapping

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Gonzalez-Jorge, S., Ha, S. H., Magallanes-Lundback, M., Ullrich, L. M., Zhou, A., Lipka, A. E., ... DellaPenna, D. (2013). Carotenoid cleavage dioxygenase4 is a negative regulator of β-carotene content in arabidopsis seeds. Plant Cell, 25(12), 4812-4826. https://doi.org/10.1105/tpc.113.119677
Gonzalez-Jorge, Sabrina ; Ha, Sun Hwa ; Magallanes-Lundback, Maria ; Ullrich, Laura M. ; Zhou, Ailing ; Lipka, Alexander E. ; Nguyen, Yen Nhu ; Angelovici, Ruthie ; Lin, Haining ; Cepela, Jason ; Little, Holly ; Buell, C. Robin ; Gore, Michael A. ; DellaPenna, Dean. / Carotenoid cleavage dioxygenase4 is a negative regulator of β-carotene content in arabidopsis seeds. In: Plant Cell. 2013 ; Vol. 25, No. 12. pp. 4812-4826.
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Gonzalez-Jorge, S, Ha, SH, Magallanes-Lundback, M, Ullrich, LM, Zhou, A, Lipka, AE, Nguyen, YN, Angelovici, R, Lin, H, Cepela, J, Little, H, Buell, CR, Gore, MA & DellaPenna, D 2013, 'Carotenoid cleavage dioxygenase4 is a negative regulator of β-carotene content in arabidopsis seeds', Plant Cell, vol. 25, no. 12, pp. 4812-4826. https://doi.org/10.1105/tpc.113.119677

Carotenoid cleavage dioxygenase4 is a negative regulator of β-carotene content in arabidopsis seeds. / Gonzalez-Jorge, Sabrina; Ha, Sun Hwa; Magallanes-Lundback, Maria; Ullrich, Laura M.; Zhou, Ailing; Lipka, Alexander E.; Nguyen, Yen Nhu; Angelovici, Ruthie; Lin, Haining; Cepela, Jason; Little, Holly; Buell, C. Robin; Gore, Michael A.; DellaPenna, Dean.

In: Plant Cell, Vol. 25, No. 12, 01.12.2013, p. 4812-4826.

Research output: Contribution to journalArticle

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T1 - Carotenoid cleavage dioxygenase4 is a negative regulator of β-carotene content in arabidopsis seeds

AU - Gonzalez-Jorge, Sabrina

AU - Ha, Sun Hwa

AU - Magallanes-Lundback, Maria

AU - Ullrich, Laura M.

AU - Zhou, Ailing

AU - Lipka, Alexander E.

AU - Nguyen, Yen Nhu

AU - Angelovici, Ruthie

AU - Lin, Haining

AU - Cepela, Jason

AU - Little, Holly

AU - Buell, C. Robin

AU - Gore, Michael A.

AU - DellaPenna, Dean

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