Carrier detection in classic hemophilia by combined measurement of immunologic (VIII AGN) and procoagulant (VIII AHF) activities

M. E. Eyster, M. B. Jones, T. Moore, L. Delli Bovi

    Research output: Contribution to journalArticle

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    Abstract

    Accurate carrier detection in classic hemophilia has been difficult because of technical problems related to the performance of both immunologic (VIII AGN) and procoagulant (VIII AHF) determinations, and statistical problems related to the analysis of these data. VIII AHF was determined by a one stage assay based on the partial thromboplastin time (PTT). VIII AGN was measured by the method of quantitative immunoelectrophoresis. The discriminant function U. = 0.67 ln (AHF) - 3.17 AGN x 10-3 was calculated for a validation group of 20 normal persons and for seven obligate carriers, and tested for accuracy of prediction on a cross validation group of seven additional normal women and ten additional obligate carriers. A U. score of ≥2.54 correctly identified 25 of 27 normal persons. Sixteen of 17 obligate carriers had U. scores below 2.54. In addition, of seven possible carriers, four were identified as normal and three as carriers. Five normal women taking oral contraceptives had disproportionately high U. scores. It is concluded that detection of carriers of classic hemophilia should be possible in the clinical laboratory by calculation of a discriminant function from combined measurements of VIII AGN and VIII AHF.

    Original languageEnglish (US)
    Pages (from-to)975-981
    Number of pages7
    JournalAmerican journal of clinical pathology
    Volume65
    Issue number6
    DOIs
    StatePublished - Jan 1 1976

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    Hemophilia A
    Immunoelectrophoresis
    Partial Thromboplastin Time
    Oral Contraceptives

    All Science Journal Classification (ASJC) codes

    • Pathology and Forensic Medicine

    Cite this

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    title = "Carrier detection in classic hemophilia by combined measurement of immunologic (VIII AGN) and procoagulant (VIII AHF) activities",
    abstract = "Accurate carrier detection in classic hemophilia has been difficult because of technical problems related to the performance of both immunologic (VIII AGN) and procoagulant (VIII AHF) determinations, and statistical problems related to the analysis of these data. VIII AHF was determined by a one stage assay based on the partial thromboplastin time (PTT). VIII AGN was measured by the method of quantitative immunoelectrophoresis. The discriminant function U. = 0.67 ln (AHF) - 3.17 AGN x 10-3 was calculated for a validation group of 20 normal persons and for seven obligate carriers, and tested for accuracy of prediction on a cross validation group of seven additional normal women and ten additional obligate carriers. A U. score of ≥2.54 correctly identified 25 of 27 normal persons. Sixteen of 17 obligate carriers had U. scores below 2.54. In addition, of seven possible carriers, four were identified as normal and three as carriers. Five normal women taking oral contraceptives had disproportionately high U. scores. It is concluded that detection of carriers of classic hemophilia should be possible in the clinical laboratory by calculation of a discriminant function from combined measurements of VIII AGN and VIII AHF.",
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    Carrier detection in classic hemophilia by combined measurement of immunologic (VIII AGN) and procoagulant (VIII AHF) activities. / Eyster, M. E.; Jones, M. B.; Moore, T.; Delli Bovi, L.

    In: American journal of clinical pathology, Vol. 65, No. 6, 01.01.1976, p. 975-981.

    Research output: Contribution to journalArticle

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    N2 - Accurate carrier detection in classic hemophilia has been difficult because of technical problems related to the performance of both immunologic (VIII AGN) and procoagulant (VIII AHF) determinations, and statistical problems related to the analysis of these data. VIII AHF was determined by a one stage assay based on the partial thromboplastin time (PTT). VIII AGN was measured by the method of quantitative immunoelectrophoresis. The discriminant function U. = 0.67 ln (AHF) - 3.17 AGN x 10-3 was calculated for a validation group of 20 normal persons and for seven obligate carriers, and tested for accuracy of prediction on a cross validation group of seven additional normal women and ten additional obligate carriers. A U. score of ≥2.54 correctly identified 25 of 27 normal persons. Sixteen of 17 obligate carriers had U. scores below 2.54. In addition, of seven possible carriers, four were identified as normal and three as carriers. Five normal women taking oral contraceptives had disproportionately high U. scores. It is concluded that detection of carriers of classic hemophilia should be possible in the clinical laboratory by calculation of a discriminant function from combined measurements of VIII AGN and VIII AHF.

    AB - Accurate carrier detection in classic hemophilia has been difficult because of technical problems related to the performance of both immunologic (VIII AGN) and procoagulant (VIII AHF) determinations, and statistical problems related to the analysis of these data. VIII AHF was determined by a one stage assay based on the partial thromboplastin time (PTT). VIII AGN was measured by the method of quantitative immunoelectrophoresis. The discriminant function U. = 0.67 ln (AHF) - 3.17 AGN x 10-3 was calculated for a validation group of 20 normal persons and for seven obligate carriers, and tested for accuracy of prediction on a cross validation group of seven additional normal women and ten additional obligate carriers. A U. score of ≥2.54 correctly identified 25 of 27 normal persons. Sixteen of 17 obligate carriers had U. scores below 2.54. In addition, of seven possible carriers, four were identified as normal and three as carriers. Five normal women taking oral contraceptives had disproportionately high U. scores. It is concluded that detection of carriers of classic hemophilia should be possible in the clinical laboratory by calculation of a discriminant function from combined measurements of VIII AGN and VIII AHF.

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