Carrot (Daucus carota) hypocotyl transformation using Agrobacterium tumefaciens

John C. Thomas, Mark Guiltinan, Silvia Bustos, Terry Thomas, Craig Nessler

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Daucus carota hypocotyl sections were transformed with Agrobacterium tumefaciens LBA4404 containing CaMV 35S promoter, β-glucuronidase coding sequence and the nopaline synthase (Nos) poly adenylation sequences in Bin 19. Sliced sterile seedling hypocotyl segments were preincubated for 2 days, co-cultivated with Agrobacterium for an additional 2 days, and then transferred to medium containing 100ug/ml of kanamycin and 400ug/ml carbenicillin. In 6 weeks kanamycin resistant calli were obtained in 5.8% of the explants from one variety. Calli were subcultured on solid medium, and in 4 weeks introduced into suspension culture. NPTII and Southern blot analysis confirmed that three selected lines were transformed with 1-3 copies of the GUSII construction. GUS activity in transformants was 5 to 250 fold over background.

Original languageEnglish (US)
Pages (from-to)354-357
Number of pages4
JournalPlant Cell Reports
Volume8
Issue number6
DOIs
StatePublished - Jun 1 1989

Fingerprint

Daucus carota
kanamycin
Agrobacterium radiobacter
carrots
hypocotyls
callus
carbenicillin
nopaline
Agrobacterium
Southern blotting
explants
promoter regions
seedlings

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

Thomas, John C. ; Guiltinan, Mark ; Bustos, Silvia ; Thomas, Terry ; Nessler, Craig. / Carrot (Daucus carota) hypocotyl transformation using Agrobacterium tumefaciens. In: Plant Cell Reports. 1989 ; Vol. 8, No. 6. pp. 354-357.
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Carrot (Daucus carota) hypocotyl transformation using Agrobacterium tumefaciens. / Thomas, John C.; Guiltinan, Mark; Bustos, Silvia; Thomas, Terry; Nessler, Craig.

In: Plant Cell Reports, Vol. 8, No. 6, 01.06.1989, p. 354-357.

Research output: Contribution to journalArticle

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