Carryover effects of acute DEHP exposure on ovarian function and oocyte developmental competence in lactating cows

Dorit Kalo, Ron Hadas, Ori Furman, Julius Ben-Ari, Yehoshua Maor, Donald George Jr Patterson, Cynthia Tomey, Zvi Roth

Research output: Contribution to journalArticle

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Abstract

We examined acute exposure of Holstein cows to di(2-ethylhexyl) phthalate (DEHP) and its carryover effects on ovarian function and oocyte developmental competence. Synchronized cows were tube-fed with water or 100 mg/kg DEHP per day for 3 days. Blood, urine and milk samples were collected before, during and after DEHP exposure to examine its clearance pattern. Ovarian follicular dynamics was monitored through an entire estrous cycle by ultrasonographic scanning. Follicular fluids were aspirated from the preovulatory follicles on days 0 and 29 of the experiment and analyzed for phthalate metabolites and estradiol concentration. The aspirated follicular fluid was used as maturation medium for in-vitro embryo production. Findings revealed that DEHP impairs the pattern of follicular development, with a prominent effect on dominant follicles. The diameter and growth rate of the first- and second- wave dominant follicles were lower (P < 0.05) in the DEHP-treated group. Estradiol concentration in the follicular fluid was lower in the DEHP-treated group than in controls, and associated with a higher number of follicular pathologies (follicle diameter >25 mm). The pattern of growth and regression of the corpus luteum differed between groups, with a lower volume in the DEHP-treated group (P < 0.05). The follicular fluid aspirated from the DEHP-treated group, but not the controls, contained 23 nM mono(2-ethylhexyl) phthalate. Culturing of cumulus oocyte complexes in the follicular fluid aspirated from DEHP-treated cows reduced the proportion of oocytes progressing to the MII stage, and the proportions of 2- to 4-cell-stage embryos (P < 0.04) and 7-day blastocysts (P < 0.06). The results describe the risk associated with acute exposure to DEHP and its deleterious carryover effects on ovarian function, nuclear maturation and oocyte developmental competence.

Original languageEnglish (US)
Article numbere0130896
JournalPloS one
Volume10
Issue number7
DOIs
StatePublished - Jul 8 2015

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phthalates
Mental Competency
Oocytes
oocytes
cows
Follicular Fluid
follicular fluid
Fluids
acute exposure
Embryonic Structures
embryo (animal)
Luteolysis
phthalic acid
Estrous Cycle
tube feeding
Blastocyst
Growth
Metabolites
follicular development
corpus luteum

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Kalo, D., Hadas, R., Furman, O., Ben-Ari, J., Maor, Y., Patterson, D. G. J., ... Roth, Z. (2015). Carryover effects of acute DEHP exposure on ovarian function and oocyte developmental competence in lactating cows. PloS one, 10(7), [e0130896]. https://doi.org/10.1371/journal.pone.0130896
Kalo, Dorit ; Hadas, Ron ; Furman, Ori ; Ben-Ari, Julius ; Maor, Yehoshua ; Patterson, Donald George Jr ; Tomey, Cynthia ; Roth, Zvi. / Carryover effects of acute DEHP exposure on ovarian function and oocyte developmental competence in lactating cows. In: PloS one. 2015 ; Vol. 10, No. 7.
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abstract = "We examined acute exposure of Holstein cows to di(2-ethylhexyl) phthalate (DEHP) and its carryover effects on ovarian function and oocyte developmental competence. Synchronized cows were tube-fed with water or 100 mg/kg DEHP per day for 3 days. Blood, urine and milk samples were collected before, during and after DEHP exposure to examine its clearance pattern. Ovarian follicular dynamics was monitored through an entire estrous cycle by ultrasonographic scanning. Follicular fluids were aspirated from the preovulatory follicles on days 0 and 29 of the experiment and analyzed for phthalate metabolites and estradiol concentration. The aspirated follicular fluid was used as maturation medium for in-vitro embryo production. Findings revealed that DEHP impairs the pattern of follicular development, with a prominent effect on dominant follicles. The diameter and growth rate of the first- and second- wave dominant follicles were lower (P < 0.05) in the DEHP-treated group. Estradiol concentration in the follicular fluid was lower in the DEHP-treated group than in controls, and associated with a higher number of follicular pathologies (follicle diameter >25 mm). The pattern of growth and regression of the corpus luteum differed between groups, with a lower volume in the DEHP-treated group (P < 0.05). The follicular fluid aspirated from the DEHP-treated group, but not the controls, contained 23 nM mono(2-ethylhexyl) phthalate. Culturing of cumulus oocyte complexes in the follicular fluid aspirated from DEHP-treated cows reduced the proportion of oocytes progressing to the MII stage, and the proportions of 2- to 4-cell-stage embryos (P < 0.04) and 7-day blastocysts (P < 0.06). The results describe the risk associated with acute exposure to DEHP and its deleterious carryover effects on ovarian function, nuclear maturation and oocyte developmental competence.",
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Kalo, D, Hadas, R, Furman, O, Ben-Ari, J, Maor, Y, Patterson, DGJ, Tomey, C & Roth, Z 2015, 'Carryover effects of acute DEHP exposure on ovarian function and oocyte developmental competence in lactating cows', PloS one, vol. 10, no. 7, e0130896. https://doi.org/10.1371/journal.pone.0130896

Carryover effects of acute DEHP exposure on ovarian function and oocyte developmental competence in lactating cows. / Kalo, Dorit; Hadas, Ron; Furman, Ori; Ben-Ari, Julius; Maor, Yehoshua; Patterson, Donald George Jr; Tomey, Cynthia; Roth, Zvi.

In: PloS one, Vol. 10, No. 7, e0130896, 08.07.2015.

Research output: Contribution to journalArticle

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T1 - Carryover effects of acute DEHP exposure on ovarian function and oocyte developmental competence in lactating cows

AU - Kalo, Dorit

AU - Hadas, Ron

AU - Furman, Ori

AU - Ben-Ari, Julius

AU - Maor, Yehoshua

AU - Patterson, Donald George Jr

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AU - Roth, Zvi

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