Ca2+ regulates reactive oxygen species production and pH during mechanosensing in arabidopsis roots

Gabriele B. Monshausen, Tatiana N. Bibikova, Manfred H. Weisenseel, Simon Gilroy

Research output: Contribution to journalArticle

222 Citations (Scopus)

Abstract

Mechanical stimulation of plants triggers a cytoplasmic Ca2+ increase that is thought to link the touch stimulus to appropriate growth responses. We found that in roots of Arabidopsis thaliana, external and endogenously generated mechanical forces consistently trigger rapid and transient increases in cytosolic Ca2+ and that the signatures of these Ca2+ transients are stimulus specific. Mechanical stimulation likewise elicited an apoplastic alkalinization and cytoplasmic acidification as well as apoplastic reactive oxygen species (ROS) production. These responses showed the same kinetics as mechanically induced Ca2+ transients and could be elicited in the absence of a mechanical stimulus by artificially increasing Ca2+ concentrations. Both pH changes and ROS production were inhibited by pretreatment with a Ca2+ channel blocker, which also inhibited mechanically induced elevations in cytosolic Ca2+. In trichoblasts of the Arabidopsis root hair defective2 mutant, which lacks a functional NADPH oxidase RBOH C, touch stimulation still triggered pH changes but not the local increase in ROS production seen in wild-type plants. Thus, mechanical stimulation likely elicits Ca2+-dependent activation of RBOH C, resulting in ROS production to the cell wall. This ROS production appears to be coordinated with intra- and extracellular pH changes through the same mechanically induced cytosolic Ca2+ transient.

Original languageEnglish (US)
Pages (from-to)2341-2356
Number of pages16
JournalPlant Cell
Volume21
Issue number8
DOIs
StatePublished - Aug 1 2009

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Arabidopsis
reactive oxygen species
Reactive Oxygen Species
calcium
Touch
touch (sensation)
NADPH Oxidase
Cell Wall
alkalinization
root hairs
acidification
Growth
Arabidopsis thaliana
pretreatment
cell walls
kinetics
mutants

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Monshausen, Gabriele B. ; Bibikova, Tatiana N. ; Weisenseel, Manfred H. ; Gilroy, Simon. / Ca2+ regulates reactive oxygen species production and pH during mechanosensing in arabidopsis roots. In: Plant Cell. 2009 ; Vol. 21, No. 8. pp. 2341-2356.
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Ca2+ regulates reactive oxygen species production and pH during mechanosensing in arabidopsis roots. / Monshausen, Gabriele B.; Bibikova, Tatiana N.; Weisenseel, Manfred H.; Gilroy, Simon.

In: Plant Cell, Vol. 21, No. 8, 01.08.2009, p. 2341-2356.

Research output: Contribution to journalArticle

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