Catechol estrogen formation by the CNS

Regional distribution of estrogen-2/4-hydroxylase activity in rat brain

Judith Weisz, W. R. Crowley

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Estrogen-2/4-hydroxylase (E-2/4-H) activity was measured by a direct product isolation assay in punch biopsy specimens obtained from nine nuclear regions from forebrain of adult male rats. Tritiated catechol estrogens were isolated from incubations of tissue with [6,7-3H]estradiol from all regions studied. The amount of 4-hydroxyestradiol (4-OH-E2) formed equaled or exceeded that of 2-hydroxyestradiol (2-OH-E2). There were significant regional differences in the amounts of catechol estrogen produced. The difference was nearly 8-fold between the arcuate-median eminence (ARC-ME) and the medial preoptic nucleus (POM), regions with the highest and lowest specific activities, respectively (37.7 ± 6.2 vs. 5.1 ± 0.7 pmol/mg protein/10 min 2-OH-E2, mean + SEM, n = 6), The supraoptic nucleus was the site of second highest concentrations of E-2/4-H activity (20.3 pmol 2-OH-E2/mg protein/10 min). Estrogen-2/4-H activity in the paraventricular (PVN) and periventricular (PERI) nuclear regions, though only about half that in the SON, was significantly greater than in the remaining brain areas (nucleus interstitialis striae terminalis, caudate, anterior hypothalamic and medial preoptic nuclei and cortex. The ARC-ME, the region with the highest E-2/4-H activity is where the dopaminergic neurones and terminals from the Gn-RH neurons are concentrated. The functions regulated by these two classes of neurones, the secretion of prolactin and gonadotrophins, respectively, have been the subject of most of the previous studies aimed at establishing the role of catechol estrogen formation in the hypothalamus. The SON and PVN, additional sites with high E-2/4-H activity, are the regions where the magnocellular, oxytocinergic and vasopressinergic neurones are concentrated. These neurones, though recognized targets of estrogens' action, have not been considered previously potential sites of catechol estrogen formation or as targets of their actions.

Original languageEnglish (US)
Pages (from-to)543-549
Number of pages7
JournalNeuroendocrinology
Volume43
Issue number5
DOIs
StatePublished - Jan 1 1986

Fingerprint

Catechol Estrogens
Neurons
Median Eminence
Preoptic Area
Brain
Estrogens
Supraoptic Nucleus
Septal Nuclei
Dopaminergic Neurons
Prosencephalon
Gonadotropins
Gonadotropin-Releasing Hormone
Prolactin
Hypothalamus
Estradiol
Proteins
Biopsy
estrogen 2-hydroxylase
hydroxide ion

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

Cite this

@article{2e11b5118c844853b3f7a96f7c08f0cb,
title = "Catechol estrogen formation by the CNS: Regional distribution of estrogen-2/4-hydroxylase activity in rat brain",
abstract = "Estrogen-2/4-hydroxylase (E-2/4-H) activity was measured by a direct product isolation assay in punch biopsy specimens obtained from nine nuclear regions from forebrain of adult male rats. Tritiated catechol estrogens were isolated from incubations of tissue with [6,7-3H]estradiol from all regions studied. The amount of 4-hydroxyestradiol (4-OH-E2) formed equaled or exceeded that of 2-hydroxyestradiol (2-OH-E2). There were significant regional differences in the amounts of catechol estrogen produced. The difference was nearly 8-fold between the arcuate-median eminence (ARC-ME) and the medial preoptic nucleus (POM), regions with the highest and lowest specific activities, respectively (37.7 ± 6.2 vs. 5.1 ± 0.7 pmol/mg protein/10 min 2-OH-E2, mean + SEM, n = 6), The supraoptic nucleus was the site of second highest concentrations of E-2/4-H activity (20.3 pmol 2-OH-E2/mg protein/10 min). Estrogen-2/4-H activity in the paraventricular (PVN) and periventricular (PERI) nuclear regions, though only about half that in the SON, was significantly greater than in the remaining brain areas (nucleus interstitialis striae terminalis, caudate, anterior hypothalamic and medial preoptic nuclei and cortex. The ARC-ME, the region with the highest E-2/4-H activity is where the dopaminergic neurones and terminals from the Gn-RH neurons are concentrated. The functions regulated by these two classes of neurones, the secretion of prolactin and gonadotrophins, respectively, have been the subject of most of the previous studies aimed at establishing the role of catechol estrogen formation in the hypothalamus. The SON and PVN, additional sites with high E-2/4-H activity, are the regions where the magnocellular, oxytocinergic and vasopressinergic neurones are concentrated. These neurones, though recognized targets of estrogens' action, have not been considered previously potential sites of catechol estrogen formation or as targets of their actions.",
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Catechol estrogen formation by the CNS : Regional distribution of estrogen-2/4-hydroxylase activity in rat brain. / Weisz, Judith; Crowley, W. R.

In: Neuroendocrinology, Vol. 43, No. 5, 01.01.1986, p. 543-549.

Research output: Contribution to journalArticle

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N2 - Estrogen-2/4-hydroxylase (E-2/4-H) activity was measured by a direct product isolation assay in punch biopsy specimens obtained from nine nuclear regions from forebrain of adult male rats. Tritiated catechol estrogens were isolated from incubations of tissue with [6,7-3H]estradiol from all regions studied. The amount of 4-hydroxyestradiol (4-OH-E2) formed equaled or exceeded that of 2-hydroxyestradiol (2-OH-E2). There were significant regional differences in the amounts of catechol estrogen produced. The difference was nearly 8-fold between the arcuate-median eminence (ARC-ME) and the medial preoptic nucleus (POM), regions with the highest and lowest specific activities, respectively (37.7 ± 6.2 vs. 5.1 ± 0.7 pmol/mg protein/10 min 2-OH-E2, mean + SEM, n = 6), The supraoptic nucleus was the site of second highest concentrations of E-2/4-H activity (20.3 pmol 2-OH-E2/mg protein/10 min). Estrogen-2/4-H activity in the paraventricular (PVN) and periventricular (PERI) nuclear regions, though only about half that in the SON, was significantly greater than in the remaining brain areas (nucleus interstitialis striae terminalis, caudate, anterior hypothalamic and medial preoptic nuclei and cortex. The ARC-ME, the region with the highest E-2/4-H activity is where the dopaminergic neurones and terminals from the Gn-RH neurons are concentrated. The functions regulated by these two classes of neurones, the secretion of prolactin and gonadotrophins, respectively, have been the subject of most of the previous studies aimed at establishing the role of catechol estrogen formation in the hypothalamus. The SON and PVN, additional sites with high E-2/4-H activity, are the regions where the magnocellular, oxytocinergic and vasopressinergic neurones are concentrated. These neurones, though recognized targets of estrogens' action, have not been considered previously potential sites of catechol estrogen formation or as targets of their actions.

AB - Estrogen-2/4-hydroxylase (E-2/4-H) activity was measured by a direct product isolation assay in punch biopsy specimens obtained from nine nuclear regions from forebrain of adult male rats. Tritiated catechol estrogens were isolated from incubations of tissue with [6,7-3H]estradiol from all regions studied. The amount of 4-hydroxyestradiol (4-OH-E2) formed equaled or exceeded that of 2-hydroxyestradiol (2-OH-E2). There were significant regional differences in the amounts of catechol estrogen produced. The difference was nearly 8-fold between the arcuate-median eminence (ARC-ME) and the medial preoptic nucleus (POM), regions with the highest and lowest specific activities, respectively (37.7 ± 6.2 vs. 5.1 ± 0.7 pmol/mg protein/10 min 2-OH-E2, mean + SEM, n = 6), The supraoptic nucleus was the site of second highest concentrations of E-2/4-H activity (20.3 pmol 2-OH-E2/mg protein/10 min). Estrogen-2/4-H activity in the paraventricular (PVN) and periventricular (PERI) nuclear regions, though only about half that in the SON, was significantly greater than in the remaining brain areas (nucleus interstitialis striae terminalis, caudate, anterior hypothalamic and medial preoptic nuclei and cortex. The ARC-ME, the region with the highest E-2/4-H activity is where the dopaminergic neurones and terminals from the Gn-RH neurons are concentrated. The functions regulated by these two classes of neurones, the secretion of prolactin and gonadotrophins, respectively, have been the subject of most of the previous studies aimed at establishing the role of catechol estrogen formation in the hypothalamus. The SON and PVN, additional sites with high E-2/4-H activity, are the regions where the magnocellular, oxytocinergic and vasopressinergic neurones are concentrated. These neurones, though recognized targets of estrogens' action, have not been considered previously potential sites of catechol estrogen formation or as targets of their actions.

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