Cathodoluminescence-emitting lipid droplets in rat testis

a study by analytical color fluorescence electron microscopy

Gang Ning, Toyoshi Fujimoto, Hirotami Koike, Kazuo Ogawa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cathodoluminescence (CL) from lipid droplets (LDs) in the rat testis was examined by analytical color fluorescence electron microscopy. The results show that (1) the Cl at wavelengths of 320 nm (CL320) and 450 nm (CL450) is derived from cholesterol esters and a mixture of lipids including vitamin A esters, respectively; (2) CL320 in the LDs of Leydig cells sharply decreases on postnatal day 21, while CL320 and CL450 in the LDs of Sertoli cells begin to be detectable; (3) the CL450-emitting LDs in seminiferous tubules, whose distributional patterns display cyclic changes during the spermatogenic cycle, are involved in spermatogenesis; and (4) the intensity of CL as well as the distributional patterns of CL-emitting LDs in testicular cells change after hypophysectomy, vitamin-A deficiency, and treatment with ethylene dimethane sulfonate and testosterone propionate. This study demonstrates that analytical color fluorescence electron microscopy is a useful tool for in-vivo observation of some specific compounds which cannot be visualized by other methods.

Original languageEnglish (US)
Pages (from-to)217-225
Number of pages9
JournalCell and Tissue Research
Volume271
Issue number2
DOIs
StatePublished - Feb 1 1993

Fingerprint

Cathodoluminescence
Fluorescence microscopy
Fluorescence Microscopy
Electron microscopy
Testis
Rats
Electron Microscopy
Color
Lipids
Vitamin A
Testosterone Propionate
Vitamin A Deficiency
Hypophysectomy
Seminiferous Tubules
Leydig Cells
Sertoli Cells
Cholesterol Esters
Spermatogenesis
Esters
Observation

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

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abstract = "Cathodoluminescence (CL) from lipid droplets (LDs) in the rat testis was examined by analytical color fluorescence electron microscopy. The results show that (1) the Cl at wavelengths of 320 nm (CL320) and 450 nm (CL450) is derived from cholesterol esters and a mixture of lipids including vitamin A esters, respectively; (2) CL320 in the LDs of Leydig cells sharply decreases on postnatal day 21, while CL320 and CL450 in the LDs of Sertoli cells begin to be detectable; (3) the CL450-emitting LDs in seminiferous tubules, whose distributional patterns display cyclic changes during the spermatogenic cycle, are involved in spermatogenesis; and (4) the intensity of CL as well as the distributional patterns of CL-emitting LDs in testicular cells change after hypophysectomy, vitamin-A deficiency, and treatment with ethylene dimethane sulfonate and testosterone propionate. This study demonstrates that analytical color fluorescence electron microscopy is a useful tool for in-vivo observation of some specific compounds which cannot be visualized by other methods.",
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Cathodoluminescence-emitting lipid droplets in rat testis : a study by analytical color fluorescence electron microscopy. / Ning, Gang; Fujimoto, Toyoshi; Koike, Hirotami; Ogawa, Kazuo.

In: Cell and Tissue Research, Vol. 271, No. 2, 01.02.1993, p. 217-225.

Research output: Contribution to journalArticle

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