Cationic and neutral amino acid transporter transcript abundances are differentially expressed in the equine intestinal tract

A. D. Woodward, S. J. Holcombe, J. P. Steibel, William Burton Staniar, C. Colvin, N. L. Trottier

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

To test the hypothesis that AA transporter transcripts are present in the large intestine and similarly expressed along the intestinal tract, mRNA abundance of candidate AA transporter genes solute carrier (SLC) family 7, member 9 (SLC7A9), SLC7A1, SLC7A8, and SLC43A1 encoding for b0,+-type AA transporter (b0,+AT), cationic AA transporter-1 (CAT-1), L-type AA transporter-2 (LAT-2), and L-type AA transporter-3 (LAT-3), respectively, was determined in small and large intestinal segments of the horse. Mucosa was collected from the equine small (jejunum and ileum) and large intestine (cecum, left ventral colon, and left dorsal colon), flash frozen in liquid nitrogen, and stored at -80°C. Messenger RNA was isolated from tissue samples, followed by manufacture of cDNA. Relative quantitative reverse transcription-PCR was conducted using the 2-ΔΔCT method, with glyceralde-hyde-3-phosphate dehydrogenase serving as the house-keeping gene. Compared with the jejunum, cationic and neutral AA transporter SLC7A9 mRNA abundance was similar in the ileum, cecum, and large intestinal segments. Compared with the jejunum, cationic AA transporter SLC7A1 mRNA abundance was similar in the il- eum and decreased in the cecum, left ventral colon, and left dorsal colon (P < 0.001). Neutral AA transporter SLC7A8 mRNA abundance decreased from the cranial to caudal end of the intestinal tract (P < 0.001). Neutral AA transporter SLC43A1 mRNA abundance was similar in the ileum and left dorsal colon and increased in the cecum (P < 0.01) and left ventral colon (P < 0.1) compared with the jejunum. Cationic and neutral AA transporter SLC7A9 mRNA abundance was similarly expressed in the large compared with small intestine, whereas cationic AA transporter SLC7A1 was of low abundance in the large intestine; neutral AA transporters SLC7A8 and SLC43A1 were differentially expressed with decreased abundance of SLC7A8 and increased abundance of SLC43A1 in the large intestine. Results indicate that the large intestine might contribute to both cationic and neutral AA uptake and absorption predominantly via transporters LAT-3 and b0,+AT.

Original languageEnglish (US)
Pages (from-to)1028-1033
Number of pages6
JournalJournal of animal science
Volume88
Issue number3
DOIs
StatePublished - Mar 1 2010

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Basic Amino Acid Transport Systems
Neutral Amino Acid Transport Systems
amino acid transporters
Horses
transporters
intestines
Large Intestine
Colon
Cecum
horses
Messenger RNA
Jejunum
Cationic Amino Acid Transporter 1
Ileum
colon
large intestine
jejunum
ileum
cecum
Essential Genes

All Science Journal Classification (ASJC) codes

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Woodward, A. D. ; Holcombe, S. J. ; Steibel, J. P. ; Staniar, William Burton ; Colvin, C. ; Trottier, N. L. / Cationic and neutral amino acid transporter transcript abundances are differentially expressed in the equine intestinal tract. In: Journal of animal science. 2010 ; Vol. 88, No. 3. pp. 1028-1033.
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Cationic and neutral amino acid transporter transcript abundances are differentially expressed in the equine intestinal tract. / Woodward, A. D.; Holcombe, S. J.; Steibel, J. P.; Staniar, William Burton; Colvin, C.; Trottier, N. L.

In: Journal of animal science, Vol. 88, No. 3, 01.03.2010, p. 1028-1033.

Research output: Contribution to journalArticle

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T1 - Cationic and neutral amino acid transporter transcript abundances are differentially expressed in the equine intestinal tract

AU - Woodward, A. D.

AU - Holcombe, S. J.

AU - Steibel, J. P.

AU - Staniar, William Burton

AU - Colvin, C.

AU - Trottier, N. L.

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