CD8 binding to MHC class I molecules is influenced by T cell maturation and glycosylation

Mark A. Daniels, Lesley Devine, Joseph D. Miller, Janice M. Moser, Aron E. Lukacher, John D. Altman, Paula Kavathas, Kristin A. Hogquist, Stephen C. Jameson

Research output: Contribution to journalArticlepeer-review

154 Scopus citations


CD8 serves both as an adhesion molecule for class I MHC molecules and as a coreceptor with the TCR for T cell activation. Here we study the developmental regulation of CD8-mediated binding to noncognate peptide/MHC ligands (i.e., those not bound by the TCR). We show that CD8's ability to bind soluble class I MHC tetramers and to mediate T cell adhesion under shear flow conditions diminishes as double-positive thymocytes mature into CD8+ T cells. Furthermore, we provide evidence that this decreased CD8 binding results from increased T cell sialylation upon T cell maturation. These data suggest that CD8's ability to interact with class I MHC is not fixed and is developmentally regulated through the T cell's glycosylation state.

Original languageEnglish (US)
Pages (from-to)1051-1061
Number of pages11
Issue number6
StatePublished - 2001

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases


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