Cell biological analyses of anther morphogenesis and pollen viability in arabidopsis and rice

Fang Chang, Zaibao Zhang, Yue Jin, Hong Ma

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Major advances have been made in recent years in our understanding of anther development through a combination of genetic studies, cell biological technologies, biochemical analysis, microarray and high-throughput sequencing-based approaches. In this chapter, we summarize the widely used protocols for pollen viability staining; the investigation of anther morphogenesis by light microscopy of semi-thin sections; TUNEL assay for programmed tapetum cell death; and laser microdissection procedures to obtain specialized cells or cell layers for carrying out transcriptomics.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages203-216
Number of pages14
DOIs
StatePublished - Jan 1 2014

Publication series

NameMethods in Molecular Biology
Volume1110
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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