Cell biological analyses of anther morphogenesis and pollen viability in arabidopsis and rice

Fang Chang, Zaibao Zhang, Yue Jin, Hong Ma

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Major advances have been made in recent years in our understanding of anther development through a combination of genetic studies, cell biological technologies, biochemical analysis, microarray and high-throughput sequencing-based approaches. In this chapter, we summarize the widely used protocols for pollen viability staining; the investigation of anther morphogenesis by light microscopy of semi-thin sections; TUNEL assay for programmed tapetum cell death; and laser microdissection procedures to obtain specialized cells or cell layers for carrying out transcriptomics.

Original languageEnglish (US)
Title of host publicationFlower Development
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages203-216
Number of pages14
ISBN (Print)9781461494072
DOIs
StatePublished - Jan 1 2014

Publication series

NameMethods in Molecular Biology
Volume1110
ISSN (Print)1064-3745

Fingerprint

Pollen
Morphogenesis
Arabidopsis
Microdissection
In Situ Nick-End Labeling
Microarray Analysis
Microscopy
Lasers
Cell Death
Staining and Labeling
Technology
Light
Oryza

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Chang, F., Zhang, Z., Jin, Y., & Ma, H. (2014). Cell biological analyses of anther morphogenesis and pollen viability in arabidopsis and rice. In Flower Development: Methods and Protocols (pp. 203-216). (Methods in Molecular Biology; Vol. 1110). Humana Press Inc.. https://doi.org/10.1007/978-1-4614-9408-9_9
Chang, Fang ; Zhang, Zaibao ; Jin, Yue ; Ma, Hong. / Cell biological analyses of anther morphogenesis and pollen viability in arabidopsis and rice. Flower Development: Methods and Protocols. Humana Press Inc., 2014. pp. 203-216 (Methods in Molecular Biology).
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Chang, F, Zhang, Z, Jin, Y & Ma, H 2014, Cell biological analyses of anther morphogenesis and pollen viability in arabidopsis and rice. in Flower Development: Methods and Protocols. Methods in Molecular Biology, vol. 1110, Humana Press Inc., pp. 203-216. https://doi.org/10.1007/978-1-4614-9408-9_9

Cell biological analyses of anther morphogenesis and pollen viability in arabidopsis and rice. / Chang, Fang; Zhang, Zaibao; Jin, Yue; Ma, Hong.

Flower Development: Methods and Protocols. Humana Press Inc., 2014. p. 203-216 (Methods in Molecular Biology; Vol. 1110).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Chang F, Zhang Z, Jin Y, Ma H. Cell biological analyses of anther morphogenesis and pollen viability in arabidopsis and rice. In Flower Development: Methods and Protocols. Humana Press Inc. 2014. p. 203-216. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4614-9408-9_9