Cell biological analyses of anther morphogenesis and pollen viability in arabidopsis and rice

Fang Chang, Zaibao Zhang, Yue Jin, Hong Ma

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations

Abstract

Major advances have been made in recent years in our understanding of anther development through a combination of genetic studies, cell biological technologies, biochemical analysis, microarray and high-throughput sequencing-based approaches. In this chapter, we summarize the widely used protocols for pollen viability staining; the investigation of anther morphogenesis by light microscopy of semi-thin sections; TUNEL assay for programmed tapetum cell death; and laser microdissection procedures to obtain specialized cells or cell layers for carrying out transcriptomics.

Original languageEnglish (US)
Title of host publicationFlower Development
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages203-216
Number of pages14
ISBN (Print)9781461494072
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1110
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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    Chang, F., Zhang, Z., Jin, Y., & Ma, H. (2014). Cell biological analyses of anther morphogenesis and pollen viability in arabidopsis and rice. In Flower Development: Methods and Protocols (pp. 203-216). (Methods in Molecular Biology; Vol. 1110). Humana Press Inc.. https://doi.org/10.1007/978-1-4614-9408-9_9