Cell Surface Display Fungal Laccase as a Renewable Biocatalyst for Degradation of Persistent Micropollutants Bisphenol A and Sulfamethoxazole

Yingying Chen, Brooke Stemple, Manish Kumar, Na Wei

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Fungal laccases have high activity in degrading various persistent organic pollutants. However, using enzymes in solution for water treatment has limitations of nonreusability, short enzyme lifetimes, and high cost of single use. In this study, we developed a new type of biocatalyst by immobilizing fungal laccase on the surface of yeast cells using synthetic biology techniques. The biocatalyst, referred to as surface display laccase (SDL), had an enzyme activity of 104 ± 3 mU/g dry cell (with 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonate (ABTS)). The SDL retained over 90% of the initial enzyme activity after 25 days storage at room temperature, while, in contrast, activity of free laccase declined to 60% of its initial activity. The SDL could be reused with high stability as it retained 74% of initial activity after eight repeated batch reactions. Proof-of-concept evaluations of the effectiveness of SDL in treating contaminants of emerging concern were performed with bisphenol A and sulfamethoxazole. Results from contaminant degradation kinetics and the effects of redox mediator amendment provided insights into the factors affecting the efficacy of the SDL system. This study reports, for the first time, the development of a surface display enzyme biocatalyst as an effective and renewable alternative for treating recalcitrant organic micropollutants.

Original languageEnglish (US)
Pages (from-to)8799-8808
Number of pages10
JournalEnvironmental Science and Technology
Volume50
Issue number16
DOIs
StatePublished - Aug 16 2016

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Sulfamethoxazole
Laccase
Display devices
Degradation
degradation
Enzymes
enzyme
Enzyme activity
enzyme activity
Impurities
pollutant
sulfonate
bisphenol A
micropollutant
Organic pollutants
yeast
Water treatment
water treatment
Yeast
Cells

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry

Cite this

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abstract = "Fungal laccases have high activity in degrading various persistent organic pollutants. However, using enzymes in solution for water treatment has limitations of nonreusability, short enzyme lifetimes, and high cost of single use. In this study, we developed a new type of biocatalyst by immobilizing fungal laccase on the surface of yeast cells using synthetic biology techniques. The biocatalyst, referred to as surface display laccase (SDL), had an enzyme activity of 104 ± 3 mU/g dry cell (with 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonate (ABTS)). The SDL retained over 90{\%} of the initial enzyme activity after 25 days storage at room temperature, while, in contrast, activity of free laccase declined to 60{\%} of its initial activity. The SDL could be reused with high stability as it retained 74{\%} of initial activity after eight repeated batch reactions. Proof-of-concept evaluations of the effectiveness of SDL in treating contaminants of emerging concern were performed with bisphenol A and sulfamethoxazole. Results from contaminant degradation kinetics and the effects of redox mediator amendment provided insights into the factors affecting the efficacy of the SDL system. This study reports, for the first time, the development of a surface display enzyme biocatalyst as an effective and renewable alternative for treating recalcitrant organic micropollutants.",
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Cell Surface Display Fungal Laccase as a Renewable Biocatalyst for Degradation of Persistent Micropollutants Bisphenol A and Sulfamethoxazole. / Chen, Yingying; Stemple, Brooke; Kumar, Manish; Wei, Na.

In: Environmental Science and Technology, Vol. 50, No. 16, 16.08.2016, p. 8799-8808.

Research output: Contribution to journalArticle

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