Cell type- and tissue context-dependent nuclear distribution of human Ago2

Nishi R. Sharma, Xiaohong Wang, Vladimir Majerciak, Masahiko Ajiro, Michael Kruhlak, Craig Meyers, Zhi Ming Zheng

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Argonaute-2 protein (Ago2), a major component of RNA-induced silencing complex (RISC), has been viewed as a cytoplasmic protein. In this study, we demonstrated by immunofluorescence confocal microscopy that Ago2 is distributed mainly as a nuclear protein in primary human foreskin keratinocytes in monolayer cultures and their derived organotypic (raft) cultures, although it exhibits only a minimal level of nuclear distribution in continuous cell lines such as HeLa and HaCaT cells. Oncogenic human papillomavirus type 16 (HPV16) or type 18 (HPV18) infection of the keratinocytes does not affect the nuclear Ago2 distribution. Examination of human tissues reveals that Ago2 exhibits primarily as a nuclear protein in skin, normal cervix, and cervical cancer tissues, but not in larynx. Together, our data provide the first convincing evidence that the subcellular distribution of Ago2 occurs in a cell type- and tissue context-dependent manner and may correlate with its various functions in regulation of gene expression.

Original languageEnglish (US)
Pages (from-to)2302-2309
Number of pages8
JournalJournal of Biological Chemistry
Volume291
Issue number5
DOIs
StatePublished - Jan 29 2016

Fingerprint

Argonaute Proteins
Tissue
Nuclear Proteins
Proteins
Keratinocytes
Uterine Cervical Neoplasms
RNA-Induced Silencing Complex
Foreskin
Human papillomavirus 16
Gene Expression Regulation
Larynx
HeLa Cells
Fluorescence Microscopy
Confocal Microscopy
Confocal microscopy
Gene expression
human EIF2C2 protein
Monolayers
Skin
Cell Line

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Sharma, N. R., Wang, X., Majerciak, V., Ajiro, M., Kruhlak, M., Meyers, C., & Zheng, Z. M. (2016). Cell type- and tissue context-dependent nuclear distribution of human Ago2. Journal of Biological Chemistry, 291(5), 2302-2309. https://doi.org/10.1074/jbc.C115.695049
Sharma, Nishi R. ; Wang, Xiaohong ; Majerciak, Vladimir ; Ajiro, Masahiko ; Kruhlak, Michael ; Meyers, Craig ; Zheng, Zhi Ming. / Cell type- and tissue context-dependent nuclear distribution of human Ago2. In: Journal of Biological Chemistry. 2016 ; Vol. 291, No. 5. pp. 2302-2309.
@article{14900bc3b22c4d4aa82af88ce6d0d663,
title = "Cell type- and tissue context-dependent nuclear distribution of human Ago2",
abstract = "Argonaute-2 protein (Ago2), a major component of RNA-induced silencing complex (RISC), has been viewed as a cytoplasmic protein. In this study, we demonstrated by immunofluorescence confocal microscopy that Ago2 is distributed mainly as a nuclear protein in primary human foreskin keratinocytes in monolayer cultures and their derived organotypic (raft) cultures, although it exhibits only a minimal level of nuclear distribution in continuous cell lines such as HeLa and HaCaT cells. Oncogenic human papillomavirus type 16 (HPV16) or type 18 (HPV18) infection of the keratinocytes does not affect the nuclear Ago2 distribution. Examination of human tissues reveals that Ago2 exhibits primarily as a nuclear protein in skin, normal cervix, and cervical cancer tissues, but not in larynx. Together, our data provide the first convincing evidence that the subcellular distribution of Ago2 occurs in a cell type- and tissue context-dependent manner and may correlate with its various functions in regulation of gene expression.",
author = "Sharma, {Nishi R.} and Xiaohong Wang and Vladimir Majerciak and Masahiko Ajiro and Michael Kruhlak and Craig Meyers and Zheng, {Zhi Ming}",
year = "2016",
month = "1",
day = "29",
doi = "10.1074/jbc.C115.695049",
language = "English (US)",
volume = "291",
pages = "2302--2309",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "5",

}

Sharma, NR, Wang, X, Majerciak, V, Ajiro, M, Kruhlak, M, Meyers, C & Zheng, ZM 2016, 'Cell type- and tissue context-dependent nuclear distribution of human Ago2', Journal of Biological Chemistry, vol. 291, no. 5, pp. 2302-2309. https://doi.org/10.1074/jbc.C115.695049

Cell type- and tissue context-dependent nuclear distribution of human Ago2. / Sharma, Nishi R.; Wang, Xiaohong; Majerciak, Vladimir; Ajiro, Masahiko; Kruhlak, Michael; Meyers, Craig; Zheng, Zhi Ming.

In: Journal of Biological Chemistry, Vol. 291, No. 5, 29.01.2016, p. 2302-2309.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cell type- and tissue context-dependent nuclear distribution of human Ago2

AU - Sharma, Nishi R.

AU - Wang, Xiaohong

AU - Majerciak, Vladimir

AU - Ajiro, Masahiko

AU - Kruhlak, Michael

AU - Meyers, Craig

AU - Zheng, Zhi Ming

PY - 2016/1/29

Y1 - 2016/1/29

N2 - Argonaute-2 protein (Ago2), a major component of RNA-induced silencing complex (RISC), has been viewed as a cytoplasmic protein. In this study, we demonstrated by immunofluorescence confocal microscopy that Ago2 is distributed mainly as a nuclear protein in primary human foreskin keratinocytes in monolayer cultures and their derived organotypic (raft) cultures, although it exhibits only a minimal level of nuclear distribution in continuous cell lines such as HeLa and HaCaT cells. Oncogenic human papillomavirus type 16 (HPV16) or type 18 (HPV18) infection of the keratinocytes does not affect the nuclear Ago2 distribution. Examination of human tissues reveals that Ago2 exhibits primarily as a nuclear protein in skin, normal cervix, and cervical cancer tissues, but not in larynx. Together, our data provide the first convincing evidence that the subcellular distribution of Ago2 occurs in a cell type- and tissue context-dependent manner and may correlate with its various functions in regulation of gene expression.

AB - Argonaute-2 protein (Ago2), a major component of RNA-induced silencing complex (RISC), has been viewed as a cytoplasmic protein. In this study, we demonstrated by immunofluorescence confocal microscopy that Ago2 is distributed mainly as a nuclear protein in primary human foreskin keratinocytes in monolayer cultures and their derived organotypic (raft) cultures, although it exhibits only a minimal level of nuclear distribution in continuous cell lines such as HeLa and HaCaT cells. Oncogenic human papillomavirus type 16 (HPV16) or type 18 (HPV18) infection of the keratinocytes does not affect the nuclear Ago2 distribution. Examination of human tissues reveals that Ago2 exhibits primarily as a nuclear protein in skin, normal cervix, and cervical cancer tissues, but not in larynx. Together, our data provide the first convincing evidence that the subcellular distribution of Ago2 occurs in a cell type- and tissue context-dependent manner and may correlate with its various functions in regulation of gene expression.

UR - http://www.scopus.com/inward/record.url?scp=84957031487&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84957031487&partnerID=8YFLogxK

U2 - 10.1074/jbc.C115.695049

DO - 10.1074/jbc.C115.695049

M3 - Article

C2 - 26699195

AN - SCOPUS:84957031487

VL - 291

SP - 2302

EP - 2309

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 5

ER -